华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2014年
2期
13-17,46
,共6页
石俊松%田存锋%许继国%张秀娟%李莉%张守全
石俊鬆%田存鋒%許繼國%張秀娟%李莉%張守全
석준송%전존봉%허계국%장수연%리리%장수전
虎%线粒体基因%系统进化树
虎%線粒體基因%繫統進化樹
호%선립체기인%계통진화수
tiger%mitochondrial gene%the phylogenetic tree
[目的]研究华南虎Panthera tigris amoyensis、东北虎P.tigris altaica和孟加拉虎P.tigris tigris之间的进化关系.[方法]采用PCR扩增结合质粒测序的方法,获得东北虎、孟加拉虎和华南虎线粒体DNA COⅠ基因750 bp序列、COⅢ基因737 bp的序列和ND4基因561 bp的序列.使用DNAStar和DNAssit软件对这些序列进行碱基组成、序列间的转换/颠换值、遗传距离等分析.选择家猫Felis catus、猎豹Acinonyx jubatus 及云豹Neofelis nebulosa 的mtDNA作为外群,利用Mega 4.0采用邻接法重建华南虎、东北虎、孟加拉虎的进化关系树.[结果和结论]研究发现东北虎、孟加拉虎和华南虎线粒体DNA COⅠ、COⅢ和ND4基因变异位点较少,且华南虎同孟加拉虎、东北虎的遗传距离要大于孟加拉虎和东北虎的遗传距离;构建了华南虎、东北虎、孟加拉虎的COⅠ、COⅢ和ND4基因的基因树,通过对基因树分析可以得东北虎和孟加拉均是由华南虎进化而来的结论.
[目的]研究華南虎Panthera tigris amoyensis、東北虎P.tigris altaica和孟加拉虎P.tigris tigris之間的進化關繫.[方法]採用PCR擴增結閤質粒測序的方法,穫得東北虎、孟加拉虎和華南虎線粒體DNA COⅠ基因750 bp序列、COⅢ基因737 bp的序列和ND4基因561 bp的序列.使用DNAStar和DNAssit軟件對這些序列進行堿基組成、序列間的轉換/顛換值、遺傳距離等分析.選擇傢貓Felis catus、獵豹Acinonyx jubatus 及雲豹Neofelis nebulosa 的mtDNA作為外群,利用Mega 4.0採用鄰接法重建華南虎、東北虎、孟加拉虎的進化關繫樹.[結果和結論]研究髮現東北虎、孟加拉虎和華南虎線粒體DNA COⅠ、COⅢ和ND4基因變異位點較少,且華南虎同孟加拉虎、東北虎的遺傳距離要大于孟加拉虎和東北虎的遺傳距離;構建瞭華南虎、東北虎、孟加拉虎的COⅠ、COⅢ和ND4基因的基因樹,通過對基因樹分析可以得東北虎和孟加拉均是由華南虎進化而來的結論.
[목적]연구화남호Panthera tigris amoyensis、동북호P.tigris altaica화맹가랍호P.tigris tigris지간적진화관계.[방법]채용PCR확증결합질립측서적방법,획득동북호、맹가랍호화화남호선립체DNA COⅠ기인750 bp서렬、COⅢ기인737 bp적서렬화ND4기인561 bp적서렬.사용DNAStar화DNAssit연건대저사서렬진행감기조성、서렬간적전환/전환치、유전거리등분석.선택가묘Felis catus、작표Acinonyx jubatus 급운표Neofelis nebulosa 적mtDNA작위외군,이용Mega 4.0채용린접법중건화남호、동북호、맹가랍호적진화관계수.[결과화결론]연구발현동북호、맹가랍호화화남호선립체DNA COⅠ、COⅢ화ND4기인변이위점교소,차화남호동맹가랍호、동북호적유전거리요대우맹가랍호화동북호적유전거리;구건료화남호、동북호、맹가랍호적COⅠ、COⅢ화ND4기인적기인수,통과대기인수분석가이득동북호화맹가랍균시유화남호진화이래적결론.
[Objective]To analyze the phylogenetic relationship of South China tiger , Panthera tigris amoyensis, Siberian tiger, P.tigris altaica and Bengal tiger, P.tigris tigris.[Method] The fragment se-quences of three mitochondrial genes were achieved by sequencing the PCR clones .These included COⅠ750 bp, COⅢ737 bp and ND4 561 bp based on the three gene sequences analyses with the DNAStar software and the DNAssit software .Their base substitutions , Kimura 2-parameter distances and the per-centage differences among three tiger subspecies were obtained .At the same time , the three genes se-quences had fewer mutation sites .In addition , the Kimura 2-parameter distances of tiger subspecies was studied, selecting the mtDNAs of cat , Felis catus, leopard, Acinonyx jubatus and clouded leopard , Neofelis nebulosa as outgroup .The Mega 4.0 was used to reconstruct the evolutionary relationship of South China tiger , Siberian tiger , Bengal tiger with a neighbor-joining method .[Result and conclusion]The results of the Kimura 2-parameter distances of tiger subspecies indicates that Siberian tiger is closer to Bengal tiger than to South China tiger .The results of the COⅠ, COⅢand ND4 genes show that Siberian tigers and Bengal tigers evolve from South China tigers .