CAJ | 학술논문

本试验旨在比较晶体色氨酸和包膜色氨酸对幼建鲤生长性能、蛋白质代谢及消化吸收能力的影响。试验选择初始体重为（7．73±0．03） g的健康幼建鲤300尾，随机分成2组（每组设3个重复，每个重复50尾），分别饲喂含晶体色氨酸和包膜色氨酸的试验饲料（色氨酸含量均为0．25％），进行为期60 d的生长试验。结果表明：包膜色氨酸组的特定生长率、蛋白质效率、蛋白质沉积率、脂肪沉积率均显著高于晶体色氨酸组（P＜0．05），而饲料系数则显著低于晶体色氨酸组（P＜0．05）。与晶体色氨酸相比，包膜色氨酸显著提高了肠长指数、肠体指数、肝体指数、肠道蛋白质含量、肝胰脏蛋白质含量以及前肠、中肠、后肠皱襞高度（P＜0．05）；显著提高了肝胰脏和肠道胰蛋白酶和脂肪酶活力（P＜0．05）；显著提高了中肠、后肠Na ＋，K ＋-ATP酶和γ-谷氨酰转肽酶（γ-GT）以及前肠、中肠、后肠碱性磷酸酶（AKP）活力（P＜0．05）。此外，包膜色氨酸组的血浆氨浓度、肝胰脏和肌肉谷丙转氨酶（ GPT）和谷草转氨酶（ GOT）活力均显著低于晶体色氨酸组（P＜0．05）。色氨酸体外释放速率结果表明晶体色氨酸和包膜色氨酸分别在15和180 min时释放完全。由此可见，与晶体色氨酸相比，包膜色氨酸体外释放速率较低，能更有效地提高幼建鲤的生长性能和消化吸收能力，有利于其体蛋白质的合成。
본시험지재비교정체색안산화포막색안산대유건리생장성능、단백질대사급소화흡수능력적영향。시험선택초시체중위（7．73±0．03） g적건강유건리300미，수궤분성2조（매조설3개중복，매개중복50미），분별사위함정체색안산화포막색안산적시험사료（색안산함량균위0．25％），진행위기60 d적생장시험。결과표명：포막색안산조적특정생장솔、단백질효솔、단백질침적솔、지방침적솔균현저고우정체색안산조（P＜0．05），이사료계수칙현저저우정체색안산조（P＜0．05）。여정체색안산상비，포막색안산현저제고료장장지수、장체지수、간체지수、장도단백질함량、간이장단백질함량이급전장、중장、후장추벽고도（P＜0．05）；현저제고료간이장화장도이단백매화지방매활력（P＜0．05）；현저제고료중장、후장Na ＋，K ＋-ATP매화γ-곡안선전태매（γ-GT）이급전장、중장、후장감성린산매（AKP）활력（P＜0．05）。차외，포막색안산조적혈장안농도、간이장화기육곡병전안매（ GPT）화곡초전안매（ GOT）활력균현저저우정체색안산조（P＜0．05）。색안산체외석방속솔결과표명정체색안산화포막색안산분별재15화180 min시석방완전。유차가견，여정체색안산상비，포막색안산체외석방속솔교저，능경유효지제고유건리적생장성능화소화흡수능력，유리우기체단백질적합성。
This experiment was conducted to compare the effects of crystalline tryptophane and coated trypto-phane on growth performance, protein metabolism and the abilities of digestion and absorption of juvenile Jian carp (Cyprinus carpio var.Jian).A total of 300 juvenile Jian carp with an initial body weight of (7.73 ± 0 .03 ) g were randomly divided into 2 groups ( with 3 replicates per group and 50 fish per replicate) , and the fish in the 2 groups had been fed experimental diets containing crystalline tryptophane and coated tryptophane for 60 days, respectively.The tryptophane content was 0.25% both for 2 experimental diets.The results showed as follows: the specific growth rate, protein efficiency ratio, protein retention rate and lipid retention rate in coated tryptophane group were significantly higher and the feed conversion ratio in coated tryptophane group was significantly lower than those in crystalline tryptophane group ( P<0 .05 ) .Compared with the crys-talline tryptophane, the coated tryptophane significantly improved the intestosomatic index, intestinal length in-dex, hepatosomatic index, intestinal protein content, hepatopancreas protein content and the fold heights of an-terior intestine, middle intestine and posterior intestine ( P <0.05 ); significantly increased the activities of trypsin and lipase in intestine and hepatopancreas ( P<0 .05 ); significantly increased the activities of Na +, K +-ATPase andγ-glutamyl transpeptidase (γ-GT) in middle intestine and posterior intestine and alkaline phos-phatase (AKP) in anterior intestine, middle intestine and posterior intestine (P<0.05).Moreover, the con-centration of plasma ammonia and the activities of glutamate-oxaloacetate transaminase ( GOT) and glutamate-pyruvate transaminase ( GPT ) in hepatopancreas and muscle in coated tryptophane group were significantly lower than those in crystalline tryptophane group ( P<0 .05 ) .The results of release rate of tryptophan in vitro showed that crystalline tryptophane and coated tryptophane were all released at 15 and 180 min, respectively. In conclusion, compared with the crystalline tryptophane, the coated tryptophane has a lower release rate;therefore, it can improve the growth performance and the abilities of digestion and absorption more effectively, which is beneficial to protein synthesis of juvenile Jian carp.