CAJ | 학술논문

受纯培养方法的限制，瘤胃壁黏附细菌群体结构和功能的研究发现相对较少，但近几年随着分子生物学和生物信息学的快速发展，尤其是16 S rDNA文库和聚合酶链反应-变性梯度凝胶电泳结合（ PCR-DGGE）的应用，瘤胃壁黏附细菌的多样性研究越来越多。本文将从瘤胃壁黏附细菌的多样性、影响因素和功能3个方面对其研究进展进行综述。
수순배양방법적한제，류위벽점부세균군체결구화공능적연구발현상대교소，단근궤년수착분자생물학화생물신식학적쾌속발전，우기시16 S rDNA문고화취합매련반응-변성제도응효전영결합（ PCR-DGGE）적응용，류위벽점부세균적다양성연구월래월다。본문장종류위벽점부세균적다양성、영향인소화공능3개방면대기연구진전진행종술。
For the limitation of isolation method, we have a relatively less knowledge of the community struc-tures and functions of rumen epithelial adherent bacteria flora.However, with the rapid development of molec-ular biology and bioinformatics, especially the application of 16 S rDNA library and polymerase chain reaction-denaturing gradient gel electrophoresis ( PCR-DGGE) , systematic study on the species and diversity of the ad-herent bacteria has made progress.This review summarized the species, diversity and influencing factors of the rumen epithelial adherent bacteria.