CAJ | 학술논문

利用454高通量测序技术对核桃（ Juglans regia）基因组进行了部分测序，并利用测得的序列进行核桃基因组微卫星特征分析。结果表明：研究共测得了104．5 Mb的核桃基因组序列，经序列组装得到了9787个微卫星重复序列。其中六碱基重复类型的重复数目最多，共5883个，占重复序列总数的60．11％；其次是单碱基重复类型（1289个）和四碱基重复类型（889个），分别占重复序列总数的13．17％和9．07％；另外二碱基重复768个，三碱基重复549个，五碱基409个，分别占重复序列总数的7．85％、5．61％和4．18％。在单碱基重复类型中，重复单元最多的为A/T；二碱基重复中，AT/TA和AG/CT为主要优势重复类型；三碱基、四碱基和六碱基重复类型中，AAN、AAAN和AAAAAN是主要的优势重复类型；五碱基重复类型中，AGATG和AAAAT 是主要的优势重复类型。在核桃微卫星序列中，A/T碱基含量丰富，而C/G碱基含量相对较少。试验还对核桃基因组微卫星的长度及各碱基重复类型的长度分化进行了研究，结果发现核桃基因组微卫星以第二类（长度＜20 bp）为主，且长碱基重复类型较短碱基重复类型长度分化程度低，稳定性高。
이용454고통량측서기술대핵도（ Juglans regia）기인조진행료부분측서，병이용측득적서렬진행핵도기인조미위성특정분석。결과표명：연구공측득료104．5 Mb적핵도기인조서렬，경서렬조장득도료9787개미위성중복서렬。기중륙감기중복류형적중복수목최다，공5883개，점중복서렬총수적60．11％；기차시단감기중복류형（1289개）화사감기중복류형（889개），분별점중복서렬총수적13．17％화9．07％；령외이감기중복768개，삼감기중복549개，오감기409개，분별점중복서렬총수적7．85％、5．61％화4．18％。재단감기중복류형중，중복단원최다적위A/T；이감기중복중，AT/TA화AG/CT위주요우세중복류형；삼감기、사감기화륙감기중복류형중，AAN、AAAN화AAAAAN시주요적우세중복류형；오감기중복류형중，AGATG화AAAAT 시주요적우세중복류형。재핵도미위성서렬중，A/T감기함량봉부，이C/G감기함량상대교소。시험환대핵도기인조미위성적장도급각감기중복류형적장도분화진행료연구，결과발현핵도기인조미위성이제이류（장도＜20 bp）위주，차장감기중복류형교단감기중복류형장도분화정도저，은정성고。
We sequenced the partially genome of Juglans regia with a 454-high-throughput sequencer and analyzed the character-istics of microsatellites based on the obtained sequences.Totally, we sequenced 104.5 Mb genome sequences of J.regia and detected 9 787 microsatellites (SSRs) in the assembled genome sequence.Among these SSRs, the hexanucleotied re-peats are the most abundant (5 883), accounting for 60.11%of the repetition sequence.Monomers (1 289) and Tetranu-cleotide (889) are the second and third abundant.There are 768 dinucleotide, 549 trinucleotide and 409 pentanucleotide, accounted for 7.85%, 5.61%and 4.18% of repetitive sequences, respectively.For the composition of microsatellites, A/T repeats are the most frequent motifs in mononucleotide repeats, and AT/TA, AG/TC repeats are the richest in dinu-cleotide repeats, while AAN, AAAN and AAAAAN repeats are dominant in tri-, tetra-and hexanucleotide repeats.For pentanucleotide, AGATG and AAAAT are the main repeats.In the microsatellite sequence repeats of Juglans, nucleotide A/T has a large content, while the C/G content is relatively small.In Juglans genome, the second type of SSRs (length<20bp ) is predominant .