CAJ | 학술논문

利用ISSR分子标记技术，对采自景东、易门、昆明的66个塔拉样品进行遗传多样性分析。结果表明：（1）从104对引物中筛选出来11对多态性明显、条带清晰、反应稳定的引物，11对引物共扩增出74条带，每个引物平均扩增出6．37条带，其中多态性条带数为5．55条，多态性条带比例为82．65％；（2）经软件分析得知，等位基因数值（Na）为1．8784±0．3291，有效位点数值（Ne）为1．4263±0．3420，基因多样性指数值（H）为0．2576±0．1773，香农指数值（I）为0．3947±0．2416，多态位点百分数为87．84％；（3）试验样品的遗传相似系数范围为0．5135～0．9865，具有较丰富的遗传多样性，聚类分析可分为6类。
이용ISSR분자표기기술，대채자경동、역문、곤명적66개탑랍양품진행유전다양성분석。결과표명：（1）종104대인물중사선출래11대다태성명현、조대청석、반응은정적인물，11대인물공확증출74조대，매개인물평균확증출6．37조대，기중다태성조대수위5．55조，다태성조대비례위82．65％；（2）경연건분석득지，등위기인수치（Na）위1．8784±0．3291，유효위점수치（Ne）위1．4263±0．3420，기인다양성지수치（H）위0．2576±0．1773，향농지수치（I）위0．3947±0．2416，다태위점백분수위87．84％；（3）시험양품적유전상사계수범위위0．5135～0．9865，구유교봉부적유전다양성，취류분석가분위6류。
An experiment was conducted to study the genetic diversity of 66 Tara samples from Jingdong , Yimen and Kunming by ISSR markers.11 polymorphic primers with obvious, clear and stable reaction are from 104 ISSR primers.There are 74 bands amplified with 11 primers.Each primer amplifies 6.37 bands on average.They contain 5.55 polymorphic bands and their polymorphism percentage is 82.65%.The numerical allele is Na=1.878 4±0.329 1, the effective site numerical is Ne=1.426 3±0.342 0, gene diversity index is H=0.257 6±0.177 3, Shannon index numerical is I=0.394 7±0.241 6, and the percentage of polymorphic loci is 87.84%.The range of the genetic similarity coefficient is 0.513 5-0.986 5 with abundant genetic diversity.66 materials can be divided into six categories by clustering methods.