CAJ | 학술논문

目的：观察左归降糖益肾方对MKR转基因2型糖尿病肾病(DN)小鼠足细胞nephrin、podocin表达的影响,探讨其作用机制。方法8周龄MKR小鼠40只随机分为4组,空白组(A组)、DN组(B组)、DN＋中药组(C组)、DN＋糖适平＋贝那普利组(D组)。B、C、D组给予高脂饮食联合右侧肾切除造模。手术4周后,各给药组给予相应药物,4周后处死小鼠。电化学法观察小鼠空腹血糖,全自动生化仪检测血肌酐(SCr)、尿素氮(BUN), ELISA 法测定尿微量白蛋白(UmAlb),RT-PCR 法检测 nephrin、podocin mRNA 表达,Western Blotting 法测定nephrin、podocin蛋白表达,电镜观测足细胞形态结构。结果与A组比较,B组小鼠空腹血糖、SCr、BUN及UmAlb均明显升高(P＜0.01),肾小球足细胞nephrin、podocin mRNA及蛋白表达明显下调(P＜0.01)。给药后,与B组比较,用药组小鼠血糖、SCr、BUN及UmAlb明显下降(P＜0.01),肾小球足细胞nephrin、podocin mRNA及蛋白表达明显上调(P＜0.01,P＜0.05)。电镜结果显示,给药组小鼠肾小球足细胞较B组明显改善。结论左归降糖益肾方通过恢复2型糖尿病肾病小鼠足细胞nephrin和podocin的表达而保护肾脏。
목적：관찰좌귀강당익신방대MKR전기인2형당뇨병신병(DN)소서족세포nephrin、podocin표체적영향,탐토기작용궤제。방법8주령MKR소서40지수궤분위4조,공백조(A조)、DN조(B조)、DN＋중약조(C조)、DN＋당괄평＋패나보리조(D조)。B、C、D조급여고지음식연합우측신절제조모。수술4주후,각급약조급여상응약물,4주후처사소서。전화학법관찰소서공복혈당,전자동생화의검측혈기항(SCr)、뇨소담(BUN), ELISA 법측정뇨미량백단백(UmAlb),RT-PCR 법검측 nephrin、podocin mRNA 표체,Western Blotting 법측정nephrin、podocin단백표체,전경관측족세포형태결구。결과여A조비교,B조소서공복혈당、SCr、BUN급UmAlb균명현승고(P＜0.01),신소구족세포nephrin、podocin mRNA급단백표체명현하조(P＜0.01)。급약후,여B조비교,용약조소서혈당、SCr、BUN급UmAlb명현하강(P＜0.01),신소구족세포nephrin、podocin mRNA급단백표체명현상조(P＜0.01,P＜0.05)。전경결과현시,급약조소서신소구족세포교B조명현개선。결론좌귀강당익신방통과회복2형당뇨병신병소서족세포nephrin화podocin적표체이보호신장。
Objective To investigate the effects of Zuogui Jiangtang Yishen decoction (ZGJTYS) on the expression of nephrin and podocin in podocyte of MKR mice with diabetic nephropathy (DN), and explore its mechanism. Methods Forty MKR mice (8 weeks old) were randomly divided into four groups as follows:negative control group (group A), DN model group (group B), ZGJTYS group (group C) and positive control group (group D, Gliquidone and benazepri). All mice from group B, C and D were received high-fat diet feed and unilateral nephrectomy. Four weeks after operation, all mice were received drug intervention, and four weeks later, all mice were put to death. The levels of UmAlb were observed by ELISA, the serum BUN and Cr by biochemical, and the FBG by electrochemical detection method. The nephrin and podocin mRNA expression were measured by RT-PCR and the protein expression by western blotting. The morphological structure changes of the podocytes were observed by transmission electron microscopes. Results As compared with group A, FBG, BUN, SCr and urine UmAlb in the mice of group B increased significantly (P<0.01), the expression level of nephrin and podocin mRNA and protein were markedly decreased (P<0.01). After intervention of drugs, all biochemical indicators above in the mice of group C and D significantly decreased (P<0.01), the expression level of nephrin and podocin mRNA and protein were markedly increased (P<0.01, P<0.05), compared with group B. The renal pathological lesions of group C and D were significantly improved compared with group B. Conclusion ZGJTYS decoction exerts reno-protective effect via reinstating nephrin and podocin expression to repair the damaged podocyte.