CAJ | 학술논문

目的：观察红芪黄酮对肺间质纤维化模型大鼠转化生长因子-β1(TGF-β1)蛋白表达及肺组织超微结构的影响,并探讨其作用机理。方法 Wistar大鼠随机分为空白组、模型组、强的松组及红芪黄酮高、中、低剂量组,采用气管内滴注博莱霉素方法建立肺纤维化模型,造模后第2日起,各给药组分别给予相应剂量药物,连续灌胃治疗,于第7、14、28日采用免疫组化法检测各组大鼠肺组织TGF-β1的含量表达,透射电镜观察第28日各组大鼠肺组织超微结构变化。结果空白组少数细支气管黏膜上皮细胞胞浆内可见少量TGF-β1蛋白表达；第7、14、28日模型组大鼠支气管、细支气管黏膜上皮细胞TGF-β1表达与空白组比较明显增强(P＜0.05),第7、14、28日红芪黄酮高、中剂量组及强的松组与模型组比较,TGF-β1表达均显著减弱(P＜0.05,P＜0.01)。第28日电镜观察模型组大鼠肺泡纵隔内胶原纤维明显增多,肺泡Ⅱ型细胞胞质线粒体肿胀,线粒体嵴断裂或消失,板层体明显减少；各给药组胶原纤维明显减少,均可改善肺泡Ⅱ型上皮细胞超微结构的异常改变。结论红芪黄酮可明显改善肺纤维化大鼠的病理损伤、减少细胞外基质沉积,可能与抑制 TGF-β1表达有关。
목적：관찰홍기황동대폐간질섬유화모형대서전화생장인자-β1(TGF-β1)단백표체급폐조직초미결구적영향,병탐토기작용궤리。방법 Wistar대서수궤분위공백조、모형조、강적송조급홍기황동고、중、저제량조,채용기관내적주박래매소방법건립폐섬유화모형,조모후제2일기,각급약조분별급여상응제량약물,련속관위치료,우제7、14、28일채용면역조화법검측각조대서폐조직TGF-β1적함량표체,투사전경관찰제28일각조대서폐조직초미결구변화。결과공백조소수세지기관점막상피세포포장내가견소량TGF-β1단백표체；제7、14、28일모형조대서지기관、세지기관점막상피세포TGF-β1표체여공백조비교명현증강(P＜0.05),제7、14、28일홍기황동고、중제량조급강적송조여모형조비교,TGF-β1표체균현저감약(P＜0.05,P＜0.01)。제28일전경관찰모형조대서폐포종격내효원섬유명현증다,폐포Ⅱ형세포포질선립체종창,선립체척단렬혹소실,판층체명현감소；각급약조효원섬유명현감소,균가개선폐포Ⅱ형상피세포초미결구적이상개변。결론홍기황동가명현개선폐섬유화대서적병리손상、감소세포외기질침적,가능여억제 TGF-β1표체유관。
Objective To observe the effects of Hedysari flavonoids on TGF-β1 and ultrastructure in lung tissue of rats with pulmonary fibrosis, and explore its mechanism. Methods Wistar rats were randomly divided into blank group, model group, prednisone group and Hedysari flavonoids high-, medium- and low-dose groups. Pulmonary fibrosis model was established by injection of bleomycin via trachea. After 24 h, rats were given drugs by gavage in all treatment groups once daily. The expression of TGF-β1 in lung tissue was detected by immunohistochemistry on d7, d14 and d28, and ultrastructure wasobserved by transmission electron microscope on d28. Results The positive expression of TGF-β1 was found in a few bronchiolar epithelial cell cytoplasm of blank group. Compared with the blank group, the positive expression of TGF-β1 on bronchial epithelial cell significantly increased (P<0.05) on d7, d14, and d28 in model group. Compared with the model group, the positive expression of TGF-β1 were significantly decreased (P<0.05, P<0.01) in Hedysari flavonoids high- and medium-dose groups and prednisone group on d7, d14 and d28. With electron microscopy, collagen fibers within the mediastinum in rat alveolar in model group were significantly increased, cell cytoplasm mitochondria was swollen in type Ⅱ epithelial cell.Mitochondrial crista was broken or disappeared, lamellar bodies significantly reduced. Collagen fibers reduced significantly in all treatment groups, which can improve and change ultrastructure on type Ⅱ epithelial cell. Conclusion Hedysari flavonoids can significantly reduce pathological damage and extracellular matrix deposition of rats with pulmonary fibrosis, which may have connection with inhibiting of TGF-β1 expression.