CAJ | 학술논문

背景：软骨组织工程支架作为软骨细胞外基质的替代物，其外形和孔结构对实现其作用和功能具有非常重要的意义。 　　目的：回顾目前若干种常用软骨组织工程中三维多孔支架的制备方法。 　　方法：由第一作者检索2000至2013年PubMed数据库，ELSEVIER SCIENCEDIRECT、万方数据库、中国知网数据库。英文检索词为“Cartilage tissue engineering；scaffolds；fabrication”，中文检索词为“软骨组织工程；制备方法；支架材料；多孔支架”。 　　结果与结论：制备软骨组织工程支架的方法有相分离/冷冻干燥法、水凝胶技术、快速成型技术、静电纺丝法、溶剂浇铸/粒子沥滤法及气体发泡法等。目前研究发现，支架中孔径的大小对组织的重建有着直接的影响，孔径为100-250μm的孔有益于骨及软骨组织的再生。通过溶液浇铸/粒子沥滤法、气体发泡法所制备的支架孔径大小在这一范围内，因此比较适合用于骨、软骨组织工程支架的构建。研究人员通常将多种方法结合起来，以期能制备出生物和力学性能方面更加仿生的组织工程多孔支架。
배경：연골조직공정지가작위연골세포외기질적체대물，기외형화공결구대실현기작용화공능구유비상중요적의의。 　　목적：회고목전약간충상용연골조직공정중삼유다공지가적제비방법。 　　방법：유제일작자검색2000지2013년PubMed수거고，ELSEVIER SCIENCEDIRECT、만방수거고、중국지망수거고。영문검색사위“Cartilage tissue engineering；scaffolds；fabrication”，중문검색사위“연골조직공정；제비방법；지가재료；다공지가”。 　　결과여결론：제비연골조직공정지가적방법유상분리/냉동간조법、수응효기술、쾌속성형기술、정전방사법、용제요주/입자력려법급기체발포법등。목전연구발현，지가중공경적대소대조직적중건유착직접적영향，공경위100-250μm적공유익우골급연골조직적재생。통과용액요주/입자력려법、기체발포법소제비적지가공경대소재저일범위내，인차비교괄합용우골、연골조직공정지가적구건。연구인원통상장다충방법결합기래，이기능제비출생물화역학성능방면경가방생적조직공정다공지가。
BACKGROUND:Cartilage tissue engineering scaffold is a substitution for extracellular matrix, and there is a great significance on the shape and pore structure of the scaffold. OBJECTIVE:To retrospectively focus on the fabrication technology of three-dimensional porous cartilage tissue engineering scaffolds. METHODS:The first author searched PubMed, ELSEVIER SCIENCEDIRECT, Wanfang and CNKI databases (2000/2013) to retrieve relevant articles about the fabrication technology tissue-engineered cartilage scaffolds. The key words were“cartilage tissue engineering;scaffolds;fabrication”in English and Chinese, respectively. RESULTS AND CONCLUSION:The fabrication technologies of three-dimensional porous cartilage tissue engineering scaffolds are as fol ows:Phase separation/freeze-drying, hydrogels, rapid prototyping manufacturing, electrospinning, solvent casting/particulate leaching, gas foaming. The current cartilage studies have demonstrated that the pore size has a significance on the regeneration of the cartilage tissue, the pore size ranging from 100-250μm al ows for the regeneration of bone and cartilage tissue. The scaffold fabricated by the solvent casting/particulate leaching and gas foaming technology at a pore size of 100-250μm is suitable for the bone and cartilage tissue regeneration. To obtain the adequate biological and mechanical properties, researchers usual y combine a variety of methods to fabricate the cartilage tissue engineering scaffolds.