CAJ | 학술논문

背景：前期实验以马铃薯为原料经交叉乳化制备了一种具有自主知识产权的专利产品多微孔多聚糖止血材料。目的：观察多微孔多聚糖止血粉应用于软组织创伤出血的止血效果。 　　方法：在实验组1家兔腹部软组织制作1条长约3 cm、深约0.5 cm的创面，吸干出血创面后将多微孔多聚糖止血粉直接喷洒于创面，用量1.0-2.0 g，以吸干出血创面后不做任何处理的家兔为对照。在实验组2家兔腹部组织切割1条长约3 cm、深约1.0 cm的创面，吸干出血创面后将多微孔多聚糖止血粉均匀喷洒于创面，用量1.0-2.0 g，以吸干出血创面后不做任何处理的家兔为对照。 　　结果与结论：多微孔多聚糖止血粉吸收血液中的水分，形成“糊状凝胶”止血痂黏附于出血创面表面达到止血效果。实验组1创面止血时间(15.25±1.04) s，实验组2创面止血时间(11.25±1.89)s，止血显效率为87.5%，有效率为100%；对照组创面止血时间大于5 min。喷洒多微孔多聚糖止血粉24 h苏木精-伊红染色显示，肌组织轻度水肿，小血管扩张，间质内可见少数散在中性粒细胞浸润；7d时炎症消退，肌组织表面轻度纤维化、完全吸收，接近正常组织，肌细胞未见明显变化。以上结果表明多微孔多聚糖止血粉可用于软组织创伤止血。
배경：전기실험이마령서위원료경교차유화제비료일충구유자주지식산권적전리산품다미공다취당지혈재료。목적：관찰다미공다취당지혈분응용우연조직창상출혈적지혈효과。 　　방법：재실험조1가토복부연조직제작1조장약3 cm、심약0.5 cm적창면，흡간출혈창면후장다미공다취당지혈분직접분쇄우창면，용량1.0-2.0 g，이흡간출혈창면후불주임하처리적가토위대조。재실험조2가토복부조직절할1조장약3 cm、심약1.0 cm적창면，흡간출혈창면후장다미공다취당지혈분균균분쇄우창면，용량1.0-2.0 g，이흡간출혈창면후불주임하처리적가토위대조。 　　결과여결론：다미공다취당지혈분흡수혈액중적수분，형성“호상응효”지혈가점부우출혈창면표면체도지혈효과。실험조1창면지혈시간(15.25±1.04) s，실험조2창면지혈시간(11.25±1.89)s，지혈현효솔위87.5%，유효솔위100%；대조조창면지혈시간대우5 min。분쇄다미공다취당지혈분24 h소목정-이홍염색현시，기조직경도수종，소혈관확장，간질내가견소수산재중성립세포침윤；7d시염증소퇴，기조직표면경도섬유화、완전흡수，접근정상조직，기세포미견명현변화。이상결과표명다미공다취당지혈분가용우연조직창상지혈。
BACKGROUND:A cross-emulsification patented product of microporous polysaccharide hemostatic powder was prepared using potatoes as raw material with independent intel ectual property rights. OBJECTIVE:To observe hemostasis effects of microporous polysaccharide hemostatic powder on soft tissue trauma. METHODS:A wound, about 3 cm long and 0.5 cm deep, was made on the abdominal soft tissue of rabbits in the experimental group 1, and then microporous polysaccharide hemostatic powder, 1.0-2.0 g, was sprayed directly on the wound. In the experimental group 2, a wound, about 3.0 cm long and 1.0 cm deep, was made on the abdominal soft tissue of rabbits, and then microporous polysaccharide hemostatic powder, 1.0-2.0 g, was sprayed directly on the wound. Another rabbits with untreated wound served as controls. RESULTS AND CONCLUSION:Microporous polysaccharide hemostatic powder formed a“pasty gel”covering the bleeding wound that achieved hemostatic effect. The bleeding time was (15.25±1.04) seconds in the experimental group 1 and (11.25±1.89) seconds in the experimental group 2. The marked effective and effective rates for hemostasis were 87.5%and 100%, respectively. In the control group, the bleeding time was more than 5 minutes. Hematoxylin-eosin staining showed mild muscle edema, vasodilatation of smal blood vessels, and few scattered endoplasmic neutrophils infiltrated at 24 hours after treatment with microporous polysaccharide hemostatic powder;til the 7th day, inflammation subsided, mild fibrosis was visible on muscle tissue surface, the hemostatic powder was completely absorbed, the wound tissue was close to the normal tissue, and there were no significant changes in muscle cells. These findings suggest that microporous polysaccharide hemostatic powder can be used for hemostasis of soft tissue trauma.