CAJ | 학술논문

背景：前期实验自行研发了一种可进行成骨及成软骨双向诱导分化的双腔搅拌式生物反应器。 　　目的：探索双腔搅拌式生物反应器的力学刺激能否提高组织工程骨软骨修复山羊膝关节缺损的效果。 　　方法：取青山羊12只，制作双侧后肢股骨内髁骨软骨缺损，随机分组，实验组与对照组缺损处均植入在双腔搅拌式生物反应器中进行成软骨、成骨诱导2周的自体骨髓间充质干细胞与β-磷酸三钙复合体，不同的是实验组将双腔搅拌式生物反应器置于磁力搅拌仪上给予力学刺激，对照组未给予力学刺激；空白对照组不做处置。植入后12，24周进行大体观察，Masson染色、Ⅱ型胶原免疫组织化学染色和组织学评分。 　　结果与结论：实验组与对照组均有新生软骨与骨组织生成，实验组修复骨缺损效果明显优于对照组(P<0.05)；空白对照组无新生软骨生成。表明通过双腔搅拌式生物反应器体外培养阶段的力学刺激，可以改善以山羊骨髓间充质干细胞为种子细胞、β-磷酸三钙为支架的组织工程骨软骨复合物对膝关节缺损的修复效果。
배경：전기실험자행연발료일충가진행성골급성연골쌍향유도분화적쌍강교반식생물반응기。 　　목적：탐색쌍강교반식생물반응기적역학자격능부제고조직공정골연골수복산양슬관절결손적효과。 　　방법：취청산양12지，제작쌍측후지고골내과골연골결손，수궤분조，실험조여대조조결손처균식입재쌍강교반식생물반응기중진행성연골、성골유도2주적자체골수간충질간세포여β-린산삼개복합체，불동적시실험조장쌍강교반식생물반응기치우자력교반의상급여역학자격，대조조미급여역학자격；공백대조조불주처치。식입후12，24주진행대체관찰，Masson염색、Ⅱ형효원면역조직화학염색화조직학평분。 　　결과여결론：실험조여대조조균유신생연골여골조직생성，실험조수복골결손효과명현우우대조조(P<0.05)；공백대조조무신생연골생성。표명통과쌍강교반식생물반응기체외배양계단적역학자격，가이개선이산양골수간충질간세포위충자세포、β-린산삼개위지가적조직공정골연골복합물대슬관절결손적수복효과。
BACKGROUND:A preliminary experiment developed a double-chamber stirred bioreactor which can carry out osteogenic and cartilage induction at the same time. OBJECTIVE:To explore the effects of double-chamber stirred bioreactor on the repair of goat knee cartilage defects with tissue-engineered cartilage. METHODS:Twelve goats were selected to make bilateral femoral condyle osteochondral defects models and randomized to three groups:experimental group, implanted with the composites ofβ-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor under mechanical stimulation;control group, implanted with the composites ofβ-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor;blank control group, without treatment. After 12 and 24 weeks of implantation, general observation, Masson staining, II col agen immunohistochemical staining and histological scoring were performed. RESULTS AND CONCLUSION:In the experimental and control groups, new cartilage tissue and bone tissue were visible, but the experimental group showed better repair effects than the control group (P<0.05). The blank control group had no cartilage formation. These findings indicate that under the mechanical stimulation by the double-chamber stirred bioreactor in vitro, the repair effect of tissue-engineered osteochondral complex on knee joint cartilage defects can be improved.