CAJ | 학술논문

背景：前期实验发现掺锶的聚磷酸钙材料对单独培养内皮细胞或成骨细胞的行为有显著促进作用。目的：观察掺锶聚磷酸钙对共培养下成骨细胞与脐静脉内皮细胞行为和功能蛋白表达的影响。 　　方法：取第3代人脐静脉内皮细胞与人成骨肉瘤细胞MG63以2∶1的浓度比接种于24孔板中，然后再分别加入掺锶聚磷酸钙、聚磷酸钙与羟基磷灰石，共培养7 d后。采用ELISA法检测细胞血管内皮生长因子、碱性成纤维细胞生长因子蛋白的表达，采用MTT法检测细胞活性。 　　结果与结论：与聚磷酸钙与羟基磷灰石相比，在掺锶聚磷酸钙表面生长的细胞呈现更加良好的形态，细胞融合生长形成单层覆盖在材料表面，并且在材料表面有一定的跨度生长，说明掺锶聚磷酸钙材料能促进内皮细胞在其上黏附、伸展，有利于细胞的生长和增殖。掺锶聚磷酸钙组细胞分泌的血管内皮生长因子、碱性成纤维细胞生长因子明显高于聚磷酸钙组和羟基磷灰石组(P <0.05)，说明掺锶聚磷酸钙可上调血管内皮生长因子、碱性成纤维细胞生长因子蛋白的表达。
배경：전기실험발현참송적취린산개재료대단독배양내피세포혹성골세포적행위유현저촉진작용。목적：관찰참송취린산개대공배양하성골세포여제정맥내피세포행위화공능단백표체적영향。 　　방법：취제3대인제정맥내피세포여인성골육류세포MG63이2∶1적농도비접충우24공판중，연후재분별가입참송취린산개、취린산개여간기린회석，공배양7 d후。채용ELISA법검측세포혈관내피생장인자、감성성섬유세포생장인자단백적표체，채용MTT법검측세포활성。 　　결과여결론：여취린산개여간기린회석상비，재참송취린산개표면생장적세포정현경가량호적형태，세포융합생장형성단층복개재재료표면，병차재재료표면유일정적과도생장，설명참송취린산개재료능촉진내피세포재기상점부、신전，유리우세포적생장화증식。참송취린산개조세포분비적혈관내피생장인자、감성성섬유세포생장인자명현고우취린산개조화간기린회석조(P <0.05)，설명참송취린산개가상조혈관내피생장인자、감성성섬유세포생장인자단백적표체。
BACKGROUND:Our previous studies have shown that strontium-doped calcium polyphosphate containing low-dose strontium appears to have a significant effect on angiogenesis-related behaviors of monocultured umbilical vein endothelial cells and osteoblasts. OBJECTIVE:To investigate the effect of strontium-doped calcium polyphosphate on angiogenesis-related behaviors of umbilical vein endothelial cells and osteoblasts co-cultured, including celladhesion, spreading, proliferation, as wel as the protein secretion of vascular endothelial growth factor and basic fibroblast growth factor from co-culture system in vitro. METHODS:Human umbilical vein endothelial cells and osteoblastic cells (MG63) were utilized in this study. cells from passage 3 were used for preparation of the cel-scaffold constructs. After placed in 24-wel plate at a ratio of 2:1, human umbilical vein endothelial cells and MG63 cells were seeded onto strontium-doped calcium polyphosphate, calcium polyphosphate and hydroxyapatite scaffolds and co-cultured for 7 days. The vascular endothelial growth factor and basic fibroblast growth factor protein levels were determined through a double ligand enzyme-linked immunosorbent assay. The colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay was performed to quantify the effect of scaffolds on cellproliferation. RESULTS AND CONCLUSION:Compared with those on calcium polyphosphate and hydroxyapatite scaffolds, cells on strontium-doped calcium polyphosphate scaffolds attached and spread better with a significantly improved cellproliferation. More importantly, the vascular endothelial growth factor and basic fibroblast growth factor expressions were significantly higher in the strontium-doped calcium polyphosphate group than the other two groups (P<0.05), indicating strontium-doped calcium polyphosphate can up-regulate levels of vascular endothelial growth factor and basic fibroblast growth factor proteins.