CAJ | 학술논문

本试验旨在研究热应激对奶山羊瘤胃黏膜紧密连接蛋白表达的影响。将8只体况良好,体重、年龄、胎次和产奶量相近,处于泌乳中后期的奶山羊随机分为对照组和热应激组,每组4只羊。饲养环境采取人工控温控湿。试验期45 d,在试验30和45 d时,采集瘤胃背囊和腹囊组织,分别采用免疫组化法、实时定量PCR法和蛋白质免疫印迹法检测其闭合蛋白( claudin )、闭锁小带蛋白1( ZO-1)蛋白的结构分布、基因mRNA表达量和蛋白表达量。结果表明：与对照组相比,热应激组(30 d)背囊和腹囊中claudin、ZO-1蛋白的信号强度和表达量均有所下降,而热应激组(45 d)下降更为严重。与对照组相比,热应激组(30 d)背囊和腹囊中claudin、ZO-1基因mRNA表达量均显著下降(P<0.05),而热应激组(45 d)则显著低于热应激组(30 d)(P<0.05)。结果提示,热应激对瘤胃黏膜的结构和完整性的破坏与瘤胃黏膜紧密连接蛋白表达量下降有关。
본시험지재연구열응격대내산양류위점막긴밀련접단백표체적영향。장8지체황량호,체중、년령、태차화산내량상근,처우비유중후기적내산양수궤분위대조조화열응격조,매조4지양。사양배경채취인공공온공습。시험기45 d,재시험30화45 d시,채집류위배낭화복낭조직,분별채용면역조화법、실시정량PCR법화단백질면역인적법검측기폐합단백( claudin )、폐쇄소대단백1( ZO-1)단백적결구분포、기인mRNA표체량화단백표체량。결과표명：여대조조상비,열응격조(30 d)배낭화복낭중claudin、ZO-1단백적신호강도화표체량균유소하강,이열응격조(45 d)하강경위엄중。여대조조상비,열응격조(30 d)배낭화복낭중claudin、ZO-1기인mRNA표체량균현저하강(P<0.05),이열응격조(45 d)칙현저저우열응격조(30 d)(P<0.05)。결과제시,열응격대류위점막적결구화완정성적파배여류위점막긴밀련접단백표체량하강유관。
This study was conducted to study the effects of heat stress ( HS) on expressions of tight junction proteins in ruminal mucosa of dairy goats. Eight healthy dietary goats with similar body weight, age, parity and milk yield at mid to late-locating period were randomly divided into control group and heat stress ( HS ) group with 4 goats in each group. Environmental temperature and humidity during feeding period were artifi-cially controlled. The experiment lasted for 45 days. Knapsack and abdominal sac samples were collected at 30 and 45 days of experiment to determine protein structural distribution, gene mRNA expression levels and pro-tein expression levels of claudin and zonula occluden 1 ( ZO-1 ) using the methods of immunohistochemisty, real-time quantitative PCR and West-bloting, respectively. The results showed as follows: compared with the control group, expression levels and signal intensity of claudin and ZO-1 proteins in knapsack and abdominal in HS group (30 days) tened to be decreased, and those in HS group (45 days) were further decresed. Com-pared with the control group, mRNA expression levels of claudin and ZO-1 genes in knapsack and abdominal in HS group (30 days) were significantly decreased (P<0. 05), and those in HS group (45 days) were sig-nificantly lower than those in HS group (30 days) (P<0. 05). In conclusion, the damage of heat stress on structure and integrity of ruminal mucosa is due to the declined expression of tight junction proteins.