CAJ | 학술논문

目的：检测二氢青蒿素（DHA）对胃癌SGC7901细胞中血管生成及相关基因表达的影响及意义。方法：不同浓度（5、10、20、40、80μmol/L）DHA作用SGC7901细胞24、48和72 h后，通过MTT法检测DHA对SGC7901细胞增殖的抑制作用。利用荧光定量RT-PCR方法检测细胞中人表皮生长因子（VEGF-C）、环氧合酶-2（COX-2）、血管细胞黏附分子-1（VCAM-1）和PTEN的mRNA的表达量；利用Western blot方法检测其蛋白表达量。结果：SGC7901细胞经过不同浓度DHA处理24 h以及50μmol/L DHA处理24、48和72 h后，SGC7901细胞增殖受到明显抑制，细胞中VEGF-C、COX-2、VCAM-1和PTEN的mRNA及蛋白的表达水平均都受到抑制；PTEN的mRNA及蛋白的表达水平则呈升高趋势。结论：DHA通过减少胃癌SGC7901细胞中VEGF-C、COX-2和VCAM-1基因的表达，促进PTEN的表达，抑制了肿瘤细胞的生长。
목적：검측이경청호소（DHA）대위암SGC7901세포중혈관생성급상관기인표체적영향급의의。방법：불동농도（5、10、20、40、80μmol/L）DHA작용SGC7901세포24、48화72 h후，통과MTT법검측DHA대SGC7901세포증식적억제작용。이용형광정량RT-PCR방법검측세포중인표피생장인자（VEGF-C）、배양합매-2（COX-2）、혈관세포점부분자-1（VCAM-1）화PTEN적mRNA적표체량；이용Western blot방법검측기단백표체량。결과：SGC7901세포경과불동농도DHA처리24 h이급50μmol/L DHA처리24、48화72 h후，SGC7901세포증식수도명현억제，세포중VEGF-C、COX-2、VCAM-1화PTEN적mRNA급단백적표체수평균도수도억제；PTEN적mRNA급단백적표체수평칙정승고추세。결론：DHA통과감소위암SGC7901세포중VEGF-C、COX-2화VCAM-1기인적표체，촉진PTEN적표체，억제료종류세포적생장。
Objective: To investigate the influence and significance of DHA on expression of angiogenesis-related genes in SGC7901 cells. Methods:SGC7901 were treated with DHA (5, 10, 20, 40, and 80μmol/l) for different times (24, 48, and 72 h), and the growth inhibition was detected by MTT. The expression of vascular endothelial growth factor (VEGF-C), cyclooxygenase-2 (COX-2) vascular cell adhesion molecule-1 (VCAM-1), and PTEN mRNA were detected by fluorescence-based quantitative poly-merase chain reaction (qRT-PCR). Their corresponding protein levels were tested by Western blot. Results:DHA significantly inhibited the growth of SGC7901 cells in a dose-and time-dependent manner (P<0.05). The expression of the angiogenesis-related genes signifi-cantly changed, as shown by RT-PCR and Western blot analyses. Compared with the control group, the expressions of VEGF-C, COX-2, and VCAM-1 were down-regulated, whereas the expressions of PTEN were up-regulated, after DHA treatment (P<0.05). Con-clusion:DHA inhibits cell growth in gastric cancer SGC7901 cells. The effect may be due to its reduction of VEGF-C, COX-2, and VCAM-1 gene expression, as well as its promotion of PTEN expression in gastric cancer cells.