山西医药杂志
山西醫藥雜誌
산서의약잡지
SHANXI MEDICAL JOURNAL
2014年
4期
363-365
,共3页
DNA拓扑异构酶类 ,Ⅱ型%宫颈肿瘤%癌前状态
DNA拓撲異構酶類 ,Ⅱ型%宮頸腫瘤%癌前狀態
DNA탁복이구매류 ,Ⅱ형%궁경종류%암전상태
DNA topoisomerases,typeⅡ%Cervic neoplasm%Precancerous conditions
目的：探讨DNA拓扑异构酶Ⅱα（TopoⅡα）蛋白在宫颈癌前病变及宫颈癌中的表达及其在宫颈癌发生发展中的作用。方法选取2007-2008年在山西省肿瘤医院行宫颈病变诊治的患者，其中包括慢性宫颈炎，低级别和高级别宫颈上皮内瘤变（CIN）和宫颈浸润性鳞癌（SCC）的患者分别21、33、30、73例，制作组织芯片，应用免疫组织化学法（SP）检测宫颈组织中TopoⅡα蛋白的表达情况。结果结果TopoⅡα蛋白在慢性宫颈炎上皮组织、CINⅠ、CINⅡ～Ⅲ、SCC中的表达率分别是5％，12％，90％，97％，表达率逐渐增加，高级别CIN和浸润性鳞癌的表达显著高于低级别CIN和正常宫颈组织，差异有统计学意义；而高级别CIN与浸润性癌之间、低级别CIN与正常宫颈上皮之间的表达差异均无统计学意义。结论 TopoⅡα蛋白表达随着宫颈癌前病变的严重程度的加重而增加，因此T o poⅡα蛋白可能参与了宫颈上皮的异常增生和恶性转变。
目的：探討DNA拓撲異構酶Ⅱα（TopoⅡα）蛋白在宮頸癌前病變及宮頸癌中的錶達及其在宮頸癌髮生髮展中的作用。方法選取2007-2008年在山西省腫瘤醫院行宮頸病變診治的患者，其中包括慢性宮頸炎，低級彆和高級彆宮頸上皮內瘤變（CIN）和宮頸浸潤性鱗癌（SCC）的患者分彆21、33、30、73例，製作組織芯片，應用免疫組織化學法（SP）檢測宮頸組織中TopoⅡα蛋白的錶達情況。結果結果TopoⅡα蛋白在慢性宮頸炎上皮組織、CINⅠ、CINⅡ～Ⅲ、SCC中的錶達率分彆是5％，12％，90％，97％，錶達率逐漸增加，高級彆CIN和浸潤性鱗癌的錶達顯著高于低級彆CIN和正常宮頸組織，差異有統計學意義；而高級彆CIN與浸潤性癌之間、低級彆CIN與正常宮頸上皮之間的錶達差異均無統計學意義。結論 TopoⅡα蛋白錶達隨著宮頸癌前病變的嚴重程度的加重而增加，因此T o poⅡα蛋白可能參與瞭宮頸上皮的異常增生和噁性轉變。
목적：탐토DNA탁복이구매Ⅱα（TopoⅡα）단백재궁경암전병변급궁경암중적표체급기재궁경암발생발전중적작용。방법선취2007-2008년재산서성종류의원행궁경병변진치적환자，기중포괄만성궁경염，저급별화고급별궁경상피내류변（CIN）화궁경침윤성린암（SCC）적환자분별21、33、30、73례，제작조직심편，응용면역조직화학법（SP）검측궁경조직중TopoⅡα단백적표체정황。결과결과TopoⅡα단백재만성궁경염상피조직、CINⅠ、CINⅡ～Ⅲ、SCC중적표체솔분별시5％，12％，90％，97％，표체솔축점증가，고급별CIN화침윤성린암적표체현저고우저급별CIN화정상궁경조직，차이유통계학의의；이고급별CIN여침윤성암지간、저급별CIN여정상궁경상피지간적표체차이균무통계학의의。결론 TopoⅡα단백표체수착궁경암전병변적엄중정도적가중이증가，인차T o poⅡα단백가능삼여료궁경상피적이상증생화악성전변。
Objective To study the expression of DNA topoisomerase Ⅱ (Topo Ⅱ α) protein in cervical precancerous lesions and cervical cancer .Methods The specimen were chosen including 21 cases of chronic cervici-tis ,33 cases of low-grade cervical intraepithelial neoplasia ,30 cases of high-grade cervical intraepithelial neoplasia and 73 cases of invasive carcinoma of cervix (SCC) during 2007-2008 in Shanxi Provincial Tumor Hospital .Tis-sue microarray and immunohistochemical method (SP) were used to detect the expression of Topo Ⅱα protein in cervical tissue .Results The positive rate of Topo Ⅱαprotein in epithelial tissue in chronic cervicitis ,CIN Ⅰ CINⅡ ~ Ⅲ and SCC is 5% ,12% ,90% ,and 97% respectively.The expression rate is gradually increased and the positive rate of high-grade CIN and invasive squamous cell carcinoma was significantly higher than the low-grade CIN and normal cervical tissues .There is statistical significance ;and there was no significant difference between the high-grade CIN expression and invasive carcinoma ;between the low-grade CIN and normal cervical epithelium . Conclusion The expression of Topo Ⅱαprotein increased with the increased severity of cervical precancerous le-sion ,so the Topo Ⅱ protein may be involved in cervical epithelial dysplasia and malignant transformation.
