CAJ | 학술논문

目的：观察三七总皂苷对肝素结合性表皮生长因子样生长因子（HB-EGF）诱导大鼠肾小球系膜细胞增殖的作用。方法：以原代培养大鼠肾小球系膜细胞为细胞模型，以400ng/mL三七总皂苷和100ng/mL HB-EGF为干预因素，实验分对照组、100ng/mL HB-EGF组和400ng/mL三七总皂苷+100ng/mL HB-EGF组，刺激系膜细胞24、36、48h，采用MTT方法，观察三七总皂苷对重组HB-EGF诱导系膜细胞增殖的作用情况。结果：HB-EGF刺激36h时，系膜细胞明显增殖，与对照组比较，P<0.05；三七总皂苷能抑制HB-EGF诱导的系膜细胞的增殖，与HB-EGF组比较，P<0.05。随着刺激时间的延长，三七总皂苷对HB-EGF诱导系膜细胞增殖的抑制作用增强，但36h与24h比较，差异无统计学意义（P>0.05）；与48h比较，差异有统计学意义（P<0.05）。结论：三七总皂苷能抑制HB-EGF诱导的系膜细胞增殖作用，且作用随着刺激时间延长而增强。三七总皂苷可能通过抑制HB-EGF对系膜细胞增殖作用而延缓肾小球硬化。
목적：관찰삼칠총조감대간소결합성표피생장인자양생장인자（HB-EGF）유도대서신소구계막세포증식적작용。방법：이원대배양대서신소구계막세포위세포모형，이400ng/mL삼칠총조감화100ng/mL HB-EGF위간예인소，실험분대조조、100ng/mL HB-EGF조화400ng/mL삼칠총조감+100ng/mL HB-EGF조，자격계막세포24、36、48h，채용MTT방법，관찰삼칠총조감대중조HB-EGF유도계막세포증식적작용정황。결과：HB-EGF자격36h시，계막세포명현증식，여대조조비교，P<0.05；삼칠총조감능억제HB-EGF유도적계막세포적증식，여HB-EGF조비교，P<0.05。수착자격시간적연장，삼칠총조감대HB-EGF유도계막세포증식적억제작용증강，단36h여24h비교，차이무통계학의의（P>0.05）；여48h비교，차이유통계학의의（P<0.05）。결론：삼칠총조감능억제HB-EGF유도적계막세포증식작용，차작용수착자격시간연장이증강。삼칠총조감가능통과억제HB-EGF대계막세포증식작용이연완신소구경화。
Objective:To investigate the influence of panax notoginseng saponins (PNS) on proliferation of mesangial cell induced by heparin-binding epidermal growth factor like growth factor(HB-EGF) and its mechanism in kidney disease. Methods:The primary cultured SD rat mesangial cell were used as cell models. The cells were divided into control group,100 ng/ml HB-EGF group,and 400 ng/ml PNS+100 ng/ml HB-EGF group,and were cultured with these solutions for 24,36,and 48 h,respectively. Cell proliferation was assessed by MTT colorimetric assay. Results:HB-EGF stimulated the proliferation of mesangial cells in 36 h (compared with control group P<0.05)but the effect was inhibited by PNS (compared with HB-EGG group P<0.05),and the effect of inhibition in 48 h was more obvious than that in 36 h(P<0.05). But no significant difference in the effect of inhibition was noted between cells treated for 36 h and 24 h (P>0.05). Conclusion:PNS can inhibit the proliferation of mesangial cells induced by HB-EGF,and the effect of inhibition gradually increases with the prolongation of the culture time. PNS may re-tard the development of glomerular sclerosis by inhibiting the proliferation of mesangial cells induced by HB-EGF.