CAJ | 학술논문

目的：建立红皮云杉针叶挥发油气相色谱（GC）-质谱（MS）指纹图谱及多指标成分含量测定分析方法。方法：采用GC-MS技术，以HP-5MS色谱柱为分析柱，对黑龙江省的10批红皮云杉针叶挥发油分别进行指纹图谱的测定和樟脑、龙脑、乙酸龙脑酯3个成分的含量测定。结果：指纹图谱中鉴定了22个共有峰，10批红皮云杉针叶挥发油的指纹图谱相似度大于0.90；红皮云杉针叶挥发油中樟脑、龙脑和乙酸龙脑酯在含量上存在一定差异。结论：系统方法学验证说明该方法可用于红皮云杉针叶的鉴别与品质评价。
목적：건립홍피운삼침협휘발유기상색보（GC）-질보（MS）지문도보급다지표성분함량측정분석방법。방법：채용GC-MS기술，이HP-5MS색보주위분석주，대흑룡강성적10비홍피운삼침협휘발유분별진행지문도보적측정화장뇌、용뇌、을산용뇌지3개성분적함량측정。결과：지문도보중감정료22개공유봉，10비홍피운삼침협휘발유적지문도보상사도대우0.90；홍피운삼침협휘발유중장뇌、용뇌화을산용뇌지재함량상존재일정차이。결론：계통방법학험증설명해방법가용우홍피운삼침협적감별여품질평개。
Objective: To establish an analysis method for the gas chromatograph-mass spectrometry ( GC-MS ) fin-gerprint and the content determination of multiple components of volatile oil in Picea koraiensis Nakai leaves . Meth-ods: Fingerprint determinations of 10 lots of volatile oil of Picea koraiensis Nakai leaves from Heilongjiang Province were conducted and the contents of borneol , bornyl acetate and camphor were determined by using GC-MS technology with HP-5MS chromatographic column . Results: About 22 common peaks were confirmed in the fingerprints and the similarities of 10 lots of volatile oil of Picea koraiensis Nakai were higher than 0 . 90 . The results of content determina-tion demonstrated that some differences existed among camphor , borneol and bornyl acetate . Conclusion: The valida-tion of systematic methodology showed that the method can be used for the identification of volatile oil of Picea ko-raiensis Nakai leaves and the evaluation of the quality of volatile oil .