CAJ | 학술논문

目的：探讨骨碎补总黄酮（Total Flavonoids of Rhizoma Drynariae，TFRD）对去卵巢骨质疏松（osteoporosis，OP）大鼠骨内谷氨酸信号的影响，以明确其通过参与大鼠骨内谷氨酸信号通路介导骨重建的可能机制。方法45只3月龄SPF级雌性SD大鼠随机分为假手术组（Sham，n=15）和去卵巢组（OVX，n=30）。采取双侧卵巢切除术复制OP大鼠模型，14周后行双能X线测定骨密度，模型复制成功，将OVX组随机分为模白组（OVX，n=15）和给药组（OVX+TFRD，n=15）。药物干预12周后处死动物，免疫组化法观察左股骨骨骺端谷氨酸（Glutamate，Glu）、代谢型Glu受体-5（metabotropic glutamate receptor 5，mGluR 5）及L-谷氨酸/L-门冬氨酸转运体（Glutamate/Aspartate transporter，EAAT 1）表达。结果 Glu和mGluR5主要分布于骨髓区细胞和紧贴骨髓腔壁的成骨细胞（osteoblasts，OB）；Glu的阳性标记在Sham、OVX和OVX+TFRD组的表达差异均无统计学意义；OVX+TFRD组代谢型受体mGluR5的阳性表达显著高于Sham组（P=0.009），而Sham组与OVX组之间的差异无统计学意义；转运子EAAT1除了大量分布于骨髓区细胞以外，还有少量表达于骨陷窝中的骨细胞，其阳性表达在Sham、OVX和OVX+TFRD组的差异均无统计学意义。结论在骨内Glu信号通路中，TFRD可显著提高代谢型受体mGluR5的表达，但尚不能认为其对Glu和转运子EAAT1有影响。
목적：탐토골쇄보총황동（Total Flavonoids of Rhizoma Drynariae，TFRD）대거란소골질소송（osteoporosis，OP）대서골내곡안산신호적영향，이명학기통과삼여대서골내곡안산신호통로개도골중건적가능궤제。방법45지3월령SPF급자성SD대서수궤분위가수술조（Sham，n=15）화거란소조（OVX，n=30）。채취쌍측란소절제술복제OP대서모형，14주후행쌍능X선측정골밀도，모형복제성공，장OVX조수궤분위모백조（OVX，n=15）화급약조（OVX+TFRD，n=15）。약물간예12주후처사동물，면역조화법관찰좌고골골후단곡안산（Glutamate，Glu）、대사형Glu수체-5（metabotropic glutamate receptor 5，mGluR 5）급L-곡안산/L-문동안산전운체（Glutamate/Aspartate transporter，EAAT 1）표체。결과 Glu화mGluR5주요분포우골수구세포화긴첩골수강벽적성골세포（osteoblasts，OB）；Glu적양성표기재Sham、OVX화OVX+TFRD조적표체차이균무통계학의의；OVX+TFRD조대사형수체mGluR5적양성표체현저고우Sham조（P=0.009），이Sham조여OVX조지간적차이무통계학의의；전운자EAAT1제료대량분포우골수구세포이외，환유소량표체우골함와중적골세포，기양성표체재Sham、OVX화OVX+TFRD조적차이균무통계학의의。결론재골내Glu신호통로중，TFRD가현저제고대사형수체mGluR5적표체，단상불능인위기대Glu화전운자EAAT1유영향。
Objective To observe the expression of Glu、mGluR 5 and EAAT 1 in bone tissues of ovariectomized osteoporotic rats and the effects of Total Flavonoids of Rhizoma Drynariae (TFRD) on it. Methods 45 SPF 3-month-old Sprague-Dawley (SD) female rats were randomly divided into sham operation (Sham, n=15) group and ovariectomized (OVX, n=30) group. The osteoporotic(OP) model was established by bilateral ovariectomy, 14 weeks later, we measured bone mineral density(BMD) by dual-energy X-ray and determined that OP model was successfully replicated, OVX group rats were then divided into OVX group (n=15) and OVX+TFRD group (n=15). The OVX+TFRD group was given TFRD for 12 weeks. Glutamate (Glu), metabotropic glutamate receptor 5 (mGluR 5), and Glutamate/Aspartate Transporter (GLAST/EAAT 1)’s expression of femur was examined in order to clarify the characteristics of bone glutamate signaling pathway and the effects of TFRD on it. Results Glu and ionotropic receptors mGluR 5 mainly distributed in bone marrow cells and osteoblasts closed to the bone marrow cavity walls. There were no significant differences in Glu expression among Sham group, OVX group and OVX+TFRD group. The mGluR 5 expression of OVX+TFRD group was significantly higher than that of Sham group and OVX group(P=0.009), while no significant difference was found between the latter two groups. In addition to large distribution in bone marrow cells, small amount of transporter EAAT 1 was noted to express in bone cells of the bone lacunae. There were no significant differences in EAAT 1 expression among the three groups. Conclusion In bone glutamate signaling pathway, this study demonstrated that TFRD could significantly improve the ionotropic receptor mGluR 5’s expression, but had no inlfuence for Glu and EAAT 1.