CAJ | 학술논문

为了降低饲料中非淀粉多糖质量抗营养因子，提高肉鸡对饲料养分的利用，本文研究了固态发酵阿魏酸酯酶的工艺及其对饲料养分利用率的影响。结果表明：在麦麸与麦糟质量比为2∶8、蛋白胨与酵母粉质量比为2∶4、含水量为40%、接种量为10%、培养时间为6天、培养温度为33℃条件下，阿魏酸酯酶的酶活达到了29.49 U/g，较未优化前提高了5.11倍。将浓缩后的酶液与溢多酶AF831添加入肉鸡饲料中，发现添加溢多酶及阿魏酸酯酶可以提高干物质、粗蛋白、粗灰分、酸性洗涤纤维等利用率，但作用不明显，对中性洗涤纤维改善效果极显著（P<0.01）。
위료강저사료중비정분다당질량항영양인자，제고육계대사료양분적이용，본문연구료고태발효아위산지매적공예급기대사료양분이용솔적영향。결과표명：재맥부여맥조질량비위2∶8、단백동여효모분질량비위2∶4、함수량위40%、접충량위10%、배양시간위6천、배양온도위33℃조건하，아위산지매적매활체도료29.49 U/g，교미우화전제고료5.11배。장농축후적매액여일다매AF831첨가입육계사료중，발현첨가일다매급아위산지매가이제고간물질、조단백、조회분、산성세조섬유등이용솔，단작용불명현，대중성세조섬유개선효과겁현저（P<0.01）。
To reduce non-starch polysaccharides anti-nutritional factor in feed and promote intake of nutrient,the solid-state fermentation technology of feruloyl esterase (FAE) and the influence on nutrient utilization rate were studied. Under optimized conditions of 2∶8 of the ratio for wheat bran and brewers’ spent grain (BSG),2∶4 of the ratio for peptone and yeast,40%of water content,10%of inoculum,6 days of incubation and 33 ℃ of incubation temperature,the activity of FAE was improved to five times compared to unoptimized conditions and the maximum activity was 29.49 U/g. The combination of FAE and Yiduoli enzyme was mixed in feed for broiler. FAE and Yiduoli enzyme could improve the utilization of dry matter,crude protein,crude ash,acidic detergent fiber,but the improvement was limited. The utilization of neutral detergent fiber increased markedly (P<0.01).