中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
13期
5984-5988
,共5页
模型,动物%兔%胆管狭窄%良性%电凝损伤
模型,動物%兔%膽管狹窄%良性%電凝損傷
모형,동물%토%담관협착%량성%전응손상
Model,animal%Rabbits%Biliary stricture%Benign%Electric coagulation injury
目的：电凝法建立兔损伤性胆管狭窄动物模型，探讨良性胆管狭窄形成机制。方法24只新西兰兔随机分为三组：空白对照组（ A组）、对照组（ B组）以及模型组（ C组）。使用Olympus针状刀以功率5W、维持2s电凝胆总管使其发生电凝损伤。术后密切观察动物一般情况；模型组动物于术前当日和术后7、14、21及28 d检验血清肝功能指标；术后14 d处死A、B两组全部动物，C组于术后14 d及28 d各处死一半，获取狭窄段胆总管，HE染色，行光镜检查；同时免疫组化SP法检测狭窄段胆管组织TGF-β1及α-SMA的表达。结果三组动物计划时间内无一例死亡，模型组动物总胆红素术后7 d已显著高于术前[（3.87±0.38）μmol/L vs．（0.53±0.38）μmol/L，P﹤0.01]，观察期内总体呈逐渐上升趋势。解剖见腹腔大量积液，胆管组织广泛黏连，胆总管损伤处明显狭窄，狭窄段以上胆管扩张，28 d胆囊亦显著增大。狭窄段胆管组织TGF-β1及α-SMA均高量表达，免疫组化最终评分有统计学差异（ P＜0.05）。结论采用电凝法电灼胆总管能够快速、安全、有效地建立兔胆总管良性狭窄模型；狭窄段胆管组织TGF-β1以及α-SMA的高量表达，提示它们在良性胆管狭窄形成过程中起着非常重要的作用。
目的：電凝法建立兔損傷性膽管狹窄動物模型，探討良性膽管狹窄形成機製。方法24隻新西蘭兔隨機分為三組：空白對照組（ A組）、對照組（ B組）以及模型組（ C組）。使用Olympus針狀刀以功率5W、維持2s電凝膽總管使其髮生電凝損傷。術後密切觀察動物一般情況；模型組動物于術前噹日和術後7、14、21及28 d檢驗血清肝功能指標；術後14 d處死A、B兩組全部動物，C組于術後14 d及28 d各處死一半，穫取狹窄段膽總管，HE染色，行光鏡檢查；同時免疫組化SP法檢測狹窄段膽管組織TGF-β1及α-SMA的錶達。結果三組動物計劃時間內無一例死亡，模型組動物總膽紅素術後7 d已顯著高于術前[（3.87±0.38）μmol/L vs．（0.53±0.38）μmol/L，P﹤0.01]，觀察期內總體呈逐漸上升趨勢。解剖見腹腔大量積液，膽管組織廣汎黏連，膽總管損傷處明顯狹窄，狹窄段以上膽管擴張，28 d膽囊亦顯著增大。狹窄段膽管組織TGF-β1及α-SMA均高量錶達，免疫組化最終評分有統計學差異（ P＜0.05）。結論採用電凝法電灼膽總管能夠快速、安全、有效地建立兔膽總管良性狹窄模型；狹窄段膽管組織TGF-β1以及α-SMA的高量錶達，提示它們在良性膽管狹窄形成過程中起著非常重要的作用。
목적：전응법건립토손상성담관협착동물모형，탐토량성담관협착형성궤제。방법24지신서란토수궤분위삼조：공백대조조（ A조）、대조조（ B조）이급모형조（ C조）。사용Olympus침상도이공솔5W、유지2s전응담총관사기발생전응손상。술후밀절관찰동물일반정황；모형조동물우술전당일화술후7、14、21급28 d검험혈청간공능지표；술후14 d처사A、B량조전부동물，C조우술후14 d급28 d각처사일반，획취협착단담총관，HE염색，행광경검사；동시면역조화SP법검측협착단담관조직TGF-β1급α-SMA적표체。결과삼조동물계화시간내무일례사망，모형조동물총담홍소술후7 d이현저고우술전[（3.87±0.38）μmol/L vs．（0.53±0.38）μmol/L，P﹤0.01]，관찰기내총체정축점상승추세。해부견복강대량적액，담관조직엄범점련，담총관손상처명현협착，협착단이상담관확장，28 d담낭역현저증대。협착단담관조직TGF-β1급α-SMA균고량표체，면역조화최종평분유통계학차이（ P＜0.05）。결론채용전응법전작담총관능구쾌속、안전、유효지건립토담총관량성협착모형；협착단담관조직TGF-β1이급α-SMA적고량표체，제시타문재량성담관협착형성과정중기착비상중요적작용。
Objective To establish a model of biliary stricture in rabbits and explore its mechanism . Methods The 24 rabbits were randomly divided into three groups:blank control group (group A,n =6),control group(group B,n=6)and experimental group(group C,n=12).The model of benign biliary stricture was made by Olympus Needle knife at 5 watt for 2 minutes.In the following 28 days,general conditions ,changes in body weight and the survival of animals were observed .Level of serum bilirubin was measured on the 1st,7th,14th,21th and 28th day after the surgery.Rabbits of group A and B and half of group C were sacrificed on 14th day,and the remainders of group C were sacrificed on the 28th day.Resected the bile duct and observed the histological changes of them .The expression of TGF-β1 and α-SMA was measured by the immunohistochemical SP method .Results All animals survived according to the schedule .In the experimental group ,the level of TBIL and DBIL was significantly increased from(3.87 ±0.38)μmol/L to(0.53 ±0.38)μmol/L(P﹤0.01)on 7th day after the surgery,and the level of serum bilirubin was on the rise on the whole .The proximal bile duct and gall bladder were also extended on 28th day. Immunohistochemistry revealed that the expression of TGF-β1 andα-SMA of the stenosis bile ducts was much higher than normal ones .Conclusion It is safe and effectively using the electric coagulation injury method to establish benign biliary stricture model in rabbits .The high expression of TGF-β1 and α-SMA in the tissue of benign biliary stricture predicts that they act as important roles in the process of benign biliary stricture .
