CAJ | 학술논문

为提高细胞工程实验教学的效果，培养学生的实验操作能力和解决问题能力，探索了适合于实验教学的具体化和可操作的植物细胞培养方案.大叶黄杨（Euonymus japonicus）和紫花地丁（Viola philippica）可作为植物细胞培养的理想实验材料；诱导愈伤组织的最适培养基为基本培养基+2，4-二氯苯氧乙酸2 mg/ L +6-苄氨基腺嘌呤0.4 mg/ L.
위제고세포공정실험교학적효과，배양학생적실험조작능력화해결문제능력，탐색료괄합우실험교학적구체화화가조작적식물세포배양방안.대협황양（Euonymus japonicus）화자화지정（Viola philippica）가작위식물세포배양적이상실험재료；유도유상조직적최괄배양기위기본배양기+2，4-이록분양을산2 mg/ L +6-변안기선표령0.4 mg/ L.
In order to improve the teaching effect of cell engineering experiment and cultivate the students′ ex-perimental operation ability and problem-solving ability,the specific and operable plant cell culture scheme suitable for experimental teaching was put into practice. The results showed that Euonymus japonicus and Viola philippica were suitable to be used as ideal experimental materials for plant cell culture. Optimal combination for callus induction was MS with 2,4-dichlomphenoxy acetic acid 2 mg / L,6-benzylaminopurine 0. 4 mg / L.