CAJ | 학술논문

目的：在该室Sysmex XE-2100血细胞分析仪已验证的单机模式复检规则基础上，结合国际血细胞复检规则41条，通过对实验数据进行分析，制定出适合该室Sysmex HST-N302XE血细胞分析流水线复检规则。方法将拟定的22条流水线复检规则导入HST-N302XE血细胞分析流水线操作电脑的Labman 4．2程序中，随机检测患者标本共500例，同时人工涂片做显微镜检查，包括细胞形态观察和人工白细胞分类，计算出真阳性率、假阳性率、真阴性率、假阴性率和涂片复检率。结果将人工显微镜镜检结果导入 HST-N302XE血细胞分析流水线操作电脑的Labman 4．2程序中进行统计分析。自动推片率（复检率）为29．2％（146/500），真阳性率为13．0％（65/500），假阳性率为16．2％（81/500），真阴性率为69．2％（346/500），假阴性率为1．6％（8/500）。结论该室血细胞分析流水线 HST-N302XE推片规则验证结果表明，假阴性率小于5％，复检率和假阳性率与文献报道基本一致，适用该室HST-N302XE血细胞分析流水线。流水线的应用能够使实验室在减少人力耗费的同时提高工作效率，保障检验质量。
목적：재해실Sysmex XE-2100혈세포분석의이험증적단궤모식복검규칙기출상，결합국제혈세포복검규칙41조，통과대실험수거진행분석，제정출괄합해실Sysmex HST-N302XE혈세포분석류수선복검규칙。방법장의정적22조류수선복검규칙도입HST-N302XE혈세포분석류수선조작전뇌적Labman 4．2정서중，수궤검측환자표본공500례，동시인공도편주현미경검사，포괄세포형태관찰화인공백세포분류，계산출진양성솔、가양성솔、진음성솔、가음성솔화도편복검솔。결과장인공현미경경검결과도입 HST-N302XE혈세포분석류수선조작전뇌적Labman 4．2정서중진행통계분석。자동추편솔（복검솔）위29．2％（146/500），진양성솔위13．0％（65/500），가양성솔위16．2％（81/500），진음성솔위69．2％（346/500），가음성솔위1．6％（8/500）。결론해실혈세포분석류수선 HST-N302XE추편규칙험증결과표명，가음성솔소우5％，복검솔화가양성솔여문헌보도기본일치，괄용해실HST-N302XE혈세포분석류수선。류수선적응용능구사실험실재감소인력모비적동시제고공작효솔，보장검험질량。
Objective To establish the suitable hematology review criteria for Sysmex HST-N302XE based on the verified criteria of Sysmex XE-2100 in an ISO15189 accredited laboratory in Chongqing .Methods A total of 500 samples were randomly detected and blood smears were reviewed including observation of cell morphology and manu-al WBC differential analysis .Results According to both the hematology review criteria suggested by international consensus group and the verified criteria of Sysmex XE-2100 by this laboratory ,22 items were presumed as the opti-mal hematology review criteria for Sysmex HST-N302 ,by which the prevalence for smear review ,the true positive ra-tio(TP),thefalsepositiveratio(FP),thetruenegativeratio(TN)andthefalsenegativeratio(FN)were29.2% (146/500) ,13 .0% (65/500) ,16 .2% (81/500) ,69 .2% (346/500) and 1 .6% (8/500) ,respectively .The validation results demonstrated that no blast cell and immature cell was missed .Conclusion The 22 items of hematology review criteria could be adaptive and efficient to assure quality of hematology results ,and enhance work efficiency and reduce unnec-essary labor consuming .These criteria for Sysmex HST-N302XE might deserve more laboratory practice .