CAJ | 학술논문

目的：制备抗人5型腺病毒（HAdV5）六邻体（Hexon）蛋白的单克隆抗体并对其进行鉴定。方法用纯化的Hexon蛋白免疫BALB/c小鼠，取其脾细胞与骨髓瘤细胞融合，经ELISA间接法筛选和克隆化培养，获得4株分泌抗 HAdV5的Hexon蛋白单克隆抗体的杂交瘤细胞。纯化获得单克隆抗体，使用ELISA 和Western blot鉴定单克隆抗体的敏感性和特异性。结果 ELISA和Western blot结果表明这4株单克隆抗体与 HAdV5的Hexon蛋白可以特异性结合。单克隆抗体株4A9-HRP标记和8D6建立的ELISA夹心法具备较高灵敏度和特异性。结论获得4株抗 HAdV5的 Hexon蛋白的单克隆抗体，为 HAdV5相关的疫苗研制和 HAdV5感染性疾病的早期快速诊断奠定了基础。
목적：제비항인5형선병독（HAdV5）륙린체（Hexon）단백적단극륭항체병대기진행감정。방법용순화적Hexon단백면역BALB/c소서，취기비세포여골수류세포융합，경ELISA간접법사선화극륭화배양，획득4주분비항 HAdV5적Hexon단백단극륭항체적잡교류세포。순화획득단극륭항체，사용ELISA 화Western blot감정단극륭항체적민감성화특이성。결과 ELISA화Western blot결과표명저4주단극륭항체여 HAdV5적Hexon단백가이특이성결합。단극륭항체주4A9-HRP표기화8D6건립적ELISA협심법구비교고령민도화특이성。결론획득4주항 HAdV5적 Hexon단백적단극륭항체，위 HAdV5상관적역묘연제화 HAdV5감염성질병적조기쾌속진단전정료기출。
Objective To obtain the monoclonal antibody against hexon protein of human adenovirus type 5 (HAdV5) .Methods BALB/c mice were immunized with purified hexon protein ,and the spleen cells of the mice were isolated and fused with myloma cells .Four hybridoma cell strains were screened by indirect ELISA and cul-tured ,and the sensitivity and specificity were analyzed by ELISA and Western blotting test .Results These hybrido-ma cells contained specific monoclonal antibodies against hexon protein of HAdV 5 as identified by ELISA and West-ern blot .Conclusion The monoclonal antibodies against hexon protein of HAdV5 with high specificity have been successfully obtained ,and these antibodies could be useful in developing assays for early diagnosis of HAdV 5 infec-tion and also in vaccine development based on HAdV5 virus .