CAJ | 학술논문

酸性氧化电解水作为一种高效杀菌剂，但因其低pH值，所以在制备和使用过程中会产生少量氯气污染、对金属材料有一定腐蚀性，影响其在农业和食品领域中应用。中性氧化电解水的 pH 值接近中性，可以克服以上缺点。该文考察了有效氯浓度相近的中性氧化电解水、84消毒液、H2O2和NaClO对卷心菜的杀菌作用，结果表明NEOW具有高效的杀菌作用，杀菌效果优于84消毒液、H2O2和NaClO，当NEOW有效氯含量为24.52 mg/L时，对大肠杆菌的杀菌效率为95.81%，杀灭对数值为1.38；当有效氯含量为63.42 mg/L时，对大肠杆菌的杀菌效率为98.92%，杀灭对数值为1.97。另外，研究了中性氧化电解水杀菌作用的影响因素，结果表明在一定范围内增加有效氯含量会提高杀菌效果，但有效氯含量过高时，杀菌效率反而降低，原因在于有效氯增高时，氧化电解水pH值增加，有效氯存在形式发生改变。在有效氯含量较高时，氧化电解水pH值的改变对杀菌效果影响不大；但当有效氯含量较低时，pH值越高，杀菌效果越差。此外，还考察了杀菌时间对杀菌效果的影响，杀菌时间增加有利于提高杀菌效率。该试验研究为中性氧化电解水的食品杀菌应用研究提供了理论依据，还通过其杀菌作用影响因素的研究揭示了其杀菌机理，提出了最优的杀菌条件。
산성양화전해수작위일충고효살균제，단인기저pH치，소이재제비화사용과정중회산생소량록기오염、대금속재료유일정부식성，영향기재농업화식품영역중응용。중성양화전해수적 pH 치접근중성，가이극복이상결점。해문고찰료유효록농도상근적중성양화전해수、84소독액、H2O2화NaClO대권심채적살균작용，결과표명NEOW구유고효적살균작용，살균효과우우84소독액、H2O2화NaClO，당NEOW유효록함량위24.52 mg/L시，대대장간균적살균효솔위95.81%，살멸대수치위1.38；당유효록함량위63.42 mg/L시，대대장간균적살균효솔위98.92%，살멸대수치위1.97。령외，연구료중성양화전해수살균작용적영향인소，결과표명재일정범위내증가유효록함량회제고살균효과，단유효록함량과고시，살균효솔반이강저，원인재우유효록증고시，양화전해수pH치증가，유효록존재형식발생개변。재유효록함량교고시，양화전해수pH치적개변대살균효과영향불대；단당유효록함량교저시，pH치월고，살균효과월차。차외，환고찰료살균시간대살균효과적영향，살균시간증가유리우제고살균효솔。해시험연구위중성양화전해수적식품살균응용연구제공료이론의거，환통과기살균작용영향인소적연구게시료기살균궤리，제출료최우적살균조건。
Electrolyzed Oxidizing Water (EOW) as a high efficient fungicide, which has a small amount of chlorine pollution and a little corrosion to metal material in the preparation and use of the process and brings fear and discomfort for people, directly influenced the application in the field of agriculture and food. But Neutral Electrolyzed Oxidizing Water (NEOW) which has a neutral pH value can overcome these shortcomings mentioned above. The sterilizing effect of NEOW, “84”disinfectant, H2O2, and NaClO with the same available chlorine concentration (ACC) were studied in this paper. The influences of the content and existing form of available chlorine, pH value, and sterilization time were systematically studied with in-situ electrochemical-ultraviolet absorption spectroscopy in this paper. The results indicated that the sterilizing effect of NEOW was better than“84”disinfectant, H2O2, and NaClO. While the available chlorine content of NEOW was 24.52 mg/L, the killing rate for Escherichia coli was 95.81%, and the killing logarithm value was 1.38. While the available chlorine content of NEOW was 63.42 mg/L, the killing rate for Escherichia coli was 98.92%, and the killing logarithm value was 1.97. But the killing rate and the killing logarithm value for Escherichia coli of“84”disinfectant (23.38 mg/L), H2O2 (21.66 mg/L), and NaClO(60.33 mg/L) were only 92.22%, 53.29%, 85.42%, and 1.11, 0.33, and 0.84. The sterilizing effect would be enhanced with the ACC increasing in a certain range. When the ACC was 10.72 mg/L, the killing rate and the killing logarithm value for Escherichia coli were 92.86%and 1.15; When the ACC was 63.42 mg/L, the killing rate and the killing logarithm value for Escherichia coli were enhanced to 98.92%and 1.97. But when the ACC was too high, such as 81.92 mg/L, the killing rate and the killing logarithm value for Escherichia coli were slightly reduced to 98.13%and 1.72. The reason lies in the pH value increase of NEOW that would lead to the available chlorine form change when the ACC increased. In the high ACC, the change of pH value had little effect on the sterilization effect. But when the ACC was low, the higher the pH value, the worse the sterilizing effect. When the pH value was 3, 5, and 7 respectively with the ACC 20.55 mg/L, the killing rate and the killing logarithm value for Escherichia coli were 96.28%, 92.68%, and 90.24%and 1.43, 1.14, and 1.01. In addition, the influence of sterilization time on the effect of sterilization was also investigated. The result shows that the increasing of sterilization time is fit to improve the efficiency of sterilization. When killing time was 2 min, 5 min, 8 min, and 10 min with the ACC 20.87 mg/L, the killing rate and the killing logarithm value for Escherichia coli were 69.83%, 77.04%, 89.80%, and 95.68%, and 0.52l L, 0.64, 0.99 and 1.36. The study provides a theoretical basis for the application of neutral electrolyzed oxidizing water. The sterilizing mechanism and optimal sterilization conditions were presented through the study of influence factors of sterilizing effect.