CAJ | 학술논문

目的观察SOXl基因过表达对鼻咽癌细胞化疗敏感性的影响.方法采用脂质体Lipofectamin2000介导的方法,将SOXl基因转入鼻咽癌细胞CNE2和HONE1中,RT-PCR和Western blot法检测转染空载体对照组及转染SOX1过表达组鼻咽癌细胞中SOX1基因的表达,并用MTT法检测两组鼻咽癌细胞对化疗药物的敏感性.结果 RT-PCR和Western blot法表明,转染过表达组鼻咽癌细胞的SOX1基因的mRNA及蛋白表达水平均较对照组明显增强,MTT法结果表明过表达SOX1的鼻咽癌细胞对化疗药物顺铂的敏感性显著提高(P＜0.05).结论转染外源性SOX1并使之在鼻咽癌细胞中过表达,能提高CNE2和HONE1细胞对化疗药物的敏感性.
목적 관찰SOXl기인과표체대비인암세포화료민감성적영향.방법 채용지질체Lipofectamin2000개도적방법,장SOXl기인전입비인암세포CNE2화HONE1중,RT-PCR화Western blot법검측전염공재체대조조급전염SOX1과표체조비인암세포중SOX1기인적표체,병용MTT법검측량조비인암세포대화료약물적민감성.결과 RT-PCR화Western blot법표명,전염과표체조비인암세포적SOX1기인적mRNA급단백표체수평균교대조조명현증강,MTT법결과표명과표체SOX1적비인암세포대화료약물순박적민감성현저제고(P＜0.05).결론 전염외원성SOX1병사지재비인암세포중과표체,능제고CNE2화HONE1세포대화료약물적민감성.
Objective To explore the effect of overexpressed SOX1 gene upon chemotherapeutic sensitivity of nasopharyngeal carcinoma cells.Methods Via Lipofectamin2000,SOX1 gene was transfected into NPC cell lines CNE2 and HONE 1.Cellular SOX1 expression was detected by reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blot analysis.MTT was employed to investigate the sensitivity of DDP treatment between NPC cells with or without SOX1 overexpression.Results RT-PCR and Western blot results demonstrated that SOX1 mRNA and protein level of CNE2-SOX1 and HONE1-SOX1 cells were significantly higher than the two NPC cell lines in control group.MTT result showed that NPC cell lines which overexpressed SOX1 were more sensitive to DDP treatment than that in control group (P ＜ 0.05).Conclusion Transfection of extrinsic SOX1 gene contributes to its overexpression in CNE2 and HONE1,which can increase the chemotherapeutic sensitivity of the two NPC cell lines.