现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
7期
152-153
,共2页
廖杰良%李拥军%熊文明%刘有添%谢水清
廖傑良%李擁軍%熊文明%劉有添%謝水清
료걸량%리옹군%웅문명%류유첨%사수청
高效液相色谱法%蔬菜%氨基甲酸酯%农药%残留量
高效液相色譜法%蔬菜%氨基甲痠酯%農藥%殘留量
고효액상색보법%소채%안기갑산지%농약%잔류량
High Performance Liquid Chromatography(HPLC)%vegetables%methylcarbamate%pesticide%residue
研究了蔬菜中的氨基甲酸酯农药(包括涕灭威、涕灭威亚砜、涕灭威砜、灭多威、3-羟基克百威、克百威和甲萘威)残留量的高效液相色谱测定方法。采用乙腈提取，经氨基固相柱层析净化后，C18柱色谱分离，采用荧光检测器检测，其激发波长为330 nm，发射波长为465 nm，7种农药在30 min内获得良好的分离，线性相关系数r为0.9998~1.0000，检测限可达11.9~24.3μg/kg，当添加水平为0.5 mg/kg时，回收率范围为75.3%~100.2%。该法灵敏度高，且易操作。
研究瞭蔬菜中的氨基甲痠酯農藥(包括涕滅威、涕滅威亞砜、涕滅威砜、滅多威、3-羥基剋百威、剋百威和甲萘威)殘留量的高效液相色譜測定方法。採用乙腈提取，經氨基固相柱層析淨化後，C18柱色譜分離，採用熒光檢測器檢測，其激髮波長為330 nm，髮射波長為465 nm，7種農藥在30 min內穫得良好的分離，線性相關繫數r為0.9998~1.0000，檢測限可達11.9~24.3μg/kg，噹添加水平為0.5 mg/kg時，迴收率範圍為75.3%~100.2%。該法靈敏度高，且易操作。
연구료소채중적안기갑산지농약(포괄체멸위、체멸위아풍、체멸위풍、멸다위、3-간기극백위、극백위화갑내위)잔류량적고효액상색보측정방법。채용을정제취，경안기고상주층석정화후，C18주색보분리，채용형광검측기검측，기격발파장위330 nm，발사파장위465 nm，7충농약재30 min내획득량호적분리，선성상관계수r위0.9998~1.0000，검측한가체11.9~24.3μg/kg，당첨가수평위0.5 mg/kg시，회수솔범위위75.3%~100.2%。해법령민도고，차역조작。
A method for determination of 7 methylcarbamate pesticides residue(including aldicarb,aldicarb sulfoxide,aldicarb sulfone,methomyl,3-hydroxycarbofuran,carbofuran and carbaryl)in vegetables by high performance liquid chromatography was investigated.The seven pesticides could be well separated within 30 min in a C18 column after pretrating of sample by extacting with acetonitrile and cleaning up with a amino solid phase extraction column.The pesticides were detected with fluorescence detector(λex=330 nm and λem=465 nm).As a spiked level was 0.5 mg/kg,the recovery of the method for the pesticides were 75.3%~100.2%,the correlation coefficient ranged from 0.999 8 to 1.000 0,the detection limits were 11.9~24.3μg/kg.The method is highly sensitive and easy in operation.
