CAJ | 학술논문

目的：探讨 survivin、caspase-3在乳腺癌细胞中的表达及与乳腺癌细胞凋亡及增殖的相关性。方法采用免疫组织化学聚合酶两步法（ PV 法）检测乳腺癌、乳腺腺瘤及乳腺正常组织中 survivin、caspase-3及 PCNA 的表达情况，TUNEL 试剂盒检测细胞凋亡情况。结果乳腺癌组织中 survivin、caspase-3表达的 OD 值、增殖指数和凋亡指数与乳腺癌及乳腺正常组织比较，差异有统计学意义（ P ＜0．05）。病理分级越低、外科分期越高，survivin 表达的 OD 值及AI 越高，caspase-3表达的 OD 值及 AI 越低（ P ＜0．05）。 survivin 蛋白的表达与乳腺癌细胞凋亡指数（AI）呈负相关（ r ＝-0．707， P ＜0．05），而与增殖指数（PI）正相关（ r ＝0．302， P ＜0．05）；caspase -3蛋白的表达与乳腺癌细胞 PI呈负相关（ r ＝-0．028， P ＜0．05），而与 AI 正相关（ r ＝0．319， P ＜0．05）；survivin 的表达与 caspase-3呈负相关（ P
목적：탐토 survivin、caspase-3재유선암세포중적표체급여유선암세포조망급증식적상관성。방법채용면역조직화학취합매량보법（ PV 법）검측유선암、유선선류급유선정상조직중 survivin、caspase-3급 PCNA 적표체정황，TUNEL 시제합검측세포조망정황。결과유선암조직중 survivin、caspase-3표체적 OD 치、증식지수화조망지수여유선암급유선정상조직비교，차이유통계학의의（ P ＜0．05）。병리분급월저、외과분기월고，survivin 표체적 OD 치급AI 월고，caspase-3표체적 OD 치급 AI 월저（ P ＜0．05）。 survivin 단백적표체여유선암세포조망지수（AI）정부상관（ r ＝-0．707， P ＜0．05），이여증식지수（PI）정상관（ r ＝0．302， P ＜0．05）；caspase -3단백적표체여유선암세포 PI정부상관（ r ＝-0．028， P ＜0．05），이여 AI 정상관（ r ＝0．319， P ＜0．05）；survivin 적표체여 caspase-3정부상관（ P
Objective To investigate the expression of survivin and caspase -3 in breast cancer cells,and to explore its correlation with apoptosis and proliferation of breast cancer .Methods Immunohistochemical method (PV) was used to detect the expressions of survivin,caspase-3 and PCNA in 300 cases of breast cancer,132 cases of breast adenoma and 103 cases of breast normal tissue.TUNEL reagent box was used to examine apoptosis cell .Results There were significant differences in the OD value of surviving,caspase-3 expression,proliferation index (PI) and apoptosis index (AI) between breast cancer tissues and breast adenoma ,breast normal tissue ( P <0.05).The lower pathological classification and the higher surgery staging of breast cancer were ,the higher OD value of surviving expression and AI ,however,the lower OD value of caspase-3 expression and AI ( P <0.05).The expression of surviving was negatively correlated to AI of breast cancer cells ( r =-0.707, P <0.05),however,which was positively correlated to PI ( r =0.302, P <0.05).The expression of caspase-3 was negatively correlated to PI of breast cancer cells ( r =-0.028, P <0.05),however,which was positively correlated to AI ( r =0.319, P <0.05).The expression of surviving was negatively related with that of caspase -3 ( P <0.05).Conclusion The cell apoptosis and disorder of cell proliferation mechanism exist generally in the pathogenesis and development of breast cancer .The abnormal expressions of survivin and caspase -3 may play an important role in the pathogenesis and development of breast cancer .