CAJ | 학술논문

稻曲病由稻曲病菌引起,是水稻穗部的重要病害.利用同源克隆和 RACE 技术,根据丝状真菌相对保守的氨基酸序列设计简并引物,从稻曲病菌中分离了丝裂原活化蛋白激酶(MAPK)编码基因的全长 cDNA 序列,命名为UvHog1. UvHog1含有 MAPK保守的蛋白激酶激活域(TGY),序列与稻瘟病菌 MgOsm1(AAF09475．1)、球孢白僵菌 BbHog1(AAS77871．1)、烟曲霉AfOsm1(XP752664．1)和隐球酵母CrHog1(AAM26267．1)等MAPK高度同源,相似性分别为95％、93％、88％和81％.系统聚类结果表明,UvHog1与酵母 Hog1 MAPK 同源.在盐胁迫条件下,UvHog1的相对表达量明显降低,表明UvHog1参与了对盐胁迫的信号响应.
도곡병유도곡병균인기,시수도수부적중요병해.이용동원극륭화 RACE 기술,근거사상진균상대보수적안기산서렬설계간병인물,종도곡병균중분리료사렬원활화단백격매(MAPK)편마기인적전장 cDNA 서렬,명명위UvHog1. UvHog1함유 MAPK보수적단백격매격활역(TGY),서렬여도온병균 MgOsm1(AAF09475．1)、구포백강균 BbHog1(AAS77871．1)、연곡매AfOsm1(XP752664．1)화은구효모CrHog1(AAM26267．1)등MAPK고도동원,상사성분별위95％、93％、88％화81％.계통취류결과표명,UvHog1여효모 Hog1 MAPK 동원.재염협박조건하,UvHog1적상대표체량명현강저,표명UvHog1삼여료대염협박적신호향응.
Rice false smut,caused by Ustilaginoidea virens,is an important disease in rice panicle.Degenerate PCR primers were designed according to the conserved amino acid sequence of several filamentous fungus mitogen activated protein kinase(MAPK)homologous to yeast Hog1 MAPK,and partial DNA fragment encoding a MAPK was amplified from U.virens through PCR.Then,a whole DNA sequence encoding the MAPK,designated as UvHog1 ,was obtained from U.virens by extending upstream and downstream sequence of the amplified fragment using RACE method.And its amino acid sequence shared 95%,93%,88% and 81% identity to MgOsm1 (AAF09475.1)from Magnaporthe grisea,BbHog1 (AAS77871.1)from Beauveria bassiana,AfOsm1 (XP752664.1)from Aspergillus fumigatus and CrHog1 (AAM26267.1 )from Cryptococcus neoformans var.neoformans,respectively.Phylogenetic clustering suggested that UvHog1 is homologous to yeast Hog1 MAPK.The results of the decreased transcript levels of UvHog1 by salinity suggest that UvHog1 may be involved in the specifically responses to salt stresses.