CAJ | 학술논문

磷转运蛋白OsPT6为水稻Pht1家族成员之一,具有吸收和转运磷酸盐双重功能.以水稻高产品种武育粳7号的OsPT6超表达转基因材料为试材,研究磷转运蛋白OsPT6在武育粳7号磷素吸收利用中的作用.结果表明：1)OsPT6在武育粳7号的地下部表达丰度较高,同时在地下部和地上部该基因均受缺磷诱导表达量显著增加.2)OsPT6超表达后,转基因植株生长良好.正常供磷和低磷处理35d后,OsPT6超表达植株地上部和地下部干物质量都显著增加.3)OsPT6超表达转基因材料在不同浓度磷素处理后,各组织全磷含量有所增加,其中营养器官尤为显著.
린전운단백OsPT6위수도Pht1가족성원지일,구유흡수화전운린산염쌍중공능.이수도고산품충무육갱7호적OsPT6초표체전기인재료위시재,연구린전운단백OsPT6재무육갱7호린소흡수이용중적작용.결과표명：1)OsPT6재무육갱7호적지하부표체봉도교고,동시재지하부화지상부해기인균수결린유도표체량현저증가.2)OsPT6초표체후,전기인식주생장량호.정상공린화저린처리35d후,OsPT6초표체식주지상부화지하부간물질량도현저증가.3)OsPT6초표체전기인재료재불동농도린소처리후,각조직전린함량유소증가,기중영양기관우위현저.
As one of members of the Pht1 transporters family in rice,the phosphate transporter OsPT6 plays a broad role in Pi uptake,translocation and internal transport throughout the plant.With the OsPT6 over-expressed transgenic rice of Wuyujing 7,a high-yielding variety,we studied the roles of OsPT6 on the phosphate uptake and utilization in rice.The main results are the following:1)OsPT6 is abundantly expressed in roots of Wuyujing 7.Furthermore,the expression levels of OsPT6 in shoots and roots are induced by phosphate deficiency.2)The over-expression of OsPT6 can promote the growth of plants.The dry weight of shoots and roots of the OsPT6 over-expressed transgenic plants was significantly increased,in both sufficient- and deficient-Pi conditions for 3 5 days.3 )The total P concentration increases in different tissues and organs of OsPT6 over-expressed transgenic plants,especially in vegetable organs,at different Pi-supply levels.