CAJ | 학술논문

目的通过研究肺癌患者组织中甲状腺转录因子-1( TTF-1)是否存在异常高表达,进而探讨其表达在不同肺癌类型之间的联系,为实现肺癌的生物学疗法提供理论依据。方法收集2009年1月至2010年12月本院经手术切除及纤维支气管镜镜下活检的43例肺癌组织及外周血标本,使用免疫组织化学S-P法研究其组织中TTF-1的表达,在表达阳性且经统计学检测TTF-1在肺癌组织者表达异常的情况下,应用RT-PCR方法进一步求证患者外周血单个核细胞中TTF-1的基因表达。结果 TTF-1在肺癌组织及外周血单个核细胞中的表达明显异常,在不同病理类型癌组织中的表达存在明显差异(字2=9.242,P=0.026<0.05),且与肺癌分化程度有关(字2=15.172,P=0.01<0.05)。结论肺癌患者癌组织TTF-1表达与肺癌有无淋巴结转移和分化程度关系密切,随病变进展及分化程度的降低而增高,可作为预测肺癌转移及预后评估的指标。
목적통과연구폐암환자조직중갑상선전록인자-1( TTF-1)시부존재이상고표체,진이탐토기표체재불동폐암류형지간적련계,위실현폐암적생물학요법제공이론의거。방법수집2009년1월지2010년12월본원경수술절제급섬유지기관경경하활검적43례폐암조직급외주혈표본,사용면역조직화학S-P법연구기조직중TTF-1적표체,재표체양성차경통계학검측TTF-1재폐암조직자표체이상적정황하,응용RT-PCR방법진일보구증환자외주혈단개핵세포중TTF-1적기인표체。결과 TTF-1재폐암조직급외주혈단개핵세포중적표체명현이상,재불동병리류형암조직중적표체존재명현차이(자2=9.242,P=0.026<0.05),차여폐암분화정도유관(자2=15.172,P=0.01<0.05)。결론폐암환자암조직TTF-1표체여폐암유무림파결전이화분화정도관계밀절,수병변진전급분화정도적강저이증고,가작위예측폐암전이급예후평고적지표。
Objective This study was aimed to evaluate the prevalence and prognostic implication of thyroid transcrip-tion factor-1(TTF-1) immunoreactivity in 43 human lung carcinomas,and to investigate its linkages between the differ-ent lung cancer types. Method From January 2009 to December 2010,after surgical resection and bronchoscopy biopsy 40 cases of lung cancer tissue and peripheral blood specimens. SP immunohistochemical method to study the expres-sion of TTF-1 in their organization,expression in ling cancer organizer application in exceptional circumstances( RT-PCR) method to further verify the statistical detection of TTF-1 expression in lung cancer organizer applications in ex-ceptional circumstances( RT-PCR) method to further verify the peripheral blood of patients with TTF-1 gene expres-sion. Result The lung cancer tissue with the degree of differentiation of lung cancer . Conclusion:the lung cancer tis-sue,TTF-1 expression in lung tissue and blood are obvious abnormality, and there are significant differences in expres-sion in lung cancer . Conclusion The lung cancer tissue,TTF-1 expression in lung cancer lymph node metastasis and closely related to the degree of differentiation, increased with disease progression and reduce the degree of differentia-tion,can be used as a predictor of lung cancer metastasis and prognosis indicatiors.