CAJ | 학술논문

目的探讨结核分枝杆菌特异性蛋白抗体检测对肺结核诊断的应用价值。方法对370例活动性肺结核患者、42例非结核性肺部疾病患者和30例健康献血员进行血清结核分枝杆菌特异性蛋白抗体检测，同时对全部肺结核和非结核性肺部疾病患者进行痰抗酸杆菌涂片和培养。结果370例临床诊断的活动性肺结核患者，结核分枝杆菌特异性蛋白抗体检测阳性284例,阳性率76.8%；42例非结核性肺部疾病患者,假阳性4例,假阳性率9.5%；30例健康献血员对照组,假阳性1例,假阳性率3.3%。检测菌阳肺结核的敏感性为81.4%,菌阴肺结核的敏感性为72.9%；阳性检出率76.8%,远高于细菌学的阳性检出率45.1%,χ2=77.72，P <0.005,具有统计学意义。特异性为93.1%。结论结核分枝杆菌特异性蛋白抗体检测对肺结核的诊断具有较好的敏感性和特异性。
목적탐토결핵분지간균특이성단백항체검측대폐결핵진단적응용개치。방법대370례활동성폐결핵환자、42례비결핵성폐부질병환자화30례건강헌혈원진행혈청결핵분지간균특이성단백항체검측，동시대전부폐결핵화비결핵성폐부질병환자진행담항산간균도편화배양。결과370례림상진단적활동성폐결핵환자，결핵분지간균특이성단백항체검측양성284례,양성솔76.8%；42례비결핵성폐부질병환자,가양성4례,가양성솔9.5%；30례건강헌혈원대조조,가양성1례,가양성솔3.3%。검측균양폐결핵적민감성위81.4%,균음폐결핵적민감성위72.9%；양성검출솔76.8%,원고우세균학적양성검출솔45.1%,χ2=77.72，P <0.005,구유통계학의의。특이성위93.1%。결론결핵분지간균특이성단백항체검측대폐결핵적진단구유교호적민감성화특이성。
Objective To evaluate the signiifcance of serum tuberculosis speciifc antigen (TB-SA) antibody detection in the diagnosis of pulmonary tuberculosis .Methods TB-SA antibody in the serum samples from370 cases of active pulmonary tuberculosis ,42 cases with non-tuberculosis pulmonary disease and 30 healthy volunteers were detected using enzyme-linked immunosorbent assay (ELISA).Results 284 of 370 active pulmonary tuberculosis patients were positive results tested by using TB-SA antibody ELISA, the positive rate was 76.8%, 4of 42 patients with other pulmonary diseases and 1of 30 healthy people were fale positive results ,the fale rate were 9.5% and 3.3% respectively. The sensitivities of TB-SA antibody in diagnosis of bacteriological positive and negative pulmonary tuberculosis patients were 81.4% and 72.9% respectively. With a total sensitivity of 76.8%, which was higher than bacteriological patients tests of 45.1%.The difference between two methods was signiifcant (P<0.005).Conclusion detection of the serum TB-SA in diagnosis of active pulmonary tuberculosis has a better sensitivity and speciifcity .