CAJ | 학술논문

采用涂布法将延胡索酸泰妙菌素生产菌种TMU10—444分离于不同平板培养基上（生产用麦芽浸膏培养基和马铃薯葡萄糖培养基），置恒温恒湿培养箱中培养成熟后，挑取单菌落、初筛、复筛，采用高效液相色谱（HPLC）法测定效价。结果表明，两种不同平板培养基上的菌落生长有差异，马铃薯葡萄糖培养基上的菌落比对照培养基上的菌落明显长得快；对马铃薯葡萄糖培养基选育的菌株进行了筛选，最终获得4支高于对照生产菌株，分别是2107、2115、2165和2183。此结论为延胡索酸泰妙菌素产生菌菌种选育提供了一种新方法。
채용도포법장연호색산태묘균소생산균충TMU10—444분리우불동평판배양기상（생산용맥아침고배양기화마령서포도당배양기），치항온항습배양상중배양성숙후，도취단균락、초사、복사，채용고효액상색보（HPLC）법측정효개。결과표명，량충불동평판배양기상적균락생장유차이，마령서포도당배양기상적균락비대조배양기상적균락명현장득쾌；대마령서포도당배양기선육적균주진행료사선，최종획득4지고우대조생산균주，분별시2107、2115、2165화2183。차결론위연호색산태묘균소산생균균충선육제공료일충신방법。
The production strain of TMII10 -444. tiamulin fumarate was coated using coating method separately in two different plating medium, i.e. malt extract medium (MEM) and potato dextrose agar medium (PDAM), under the craft conditions of constant temperature and humidity in the culture box until maturation. Then picking out a single colony, screening, and re - screening. HPLC method was used to measure chemical titer. There was difference of plate colony growth in observed two different culture mediums. The colony on PDAM grew obviously faster than that on MEM. The breeding strains were screened and selected on PDAM. There were four strains eventually selected out by comparing their higher yield titers. These four strains were 2107, 2115, 2165, and 2183, respectively.