CAJ | 학술논문

建立了高效液相色谱法测定禽、羊组织中环丙氨嗪残留量的方法。动物组织样品中残留的环丙氨嗪经三氯乙酸／乙腈溶液提取，MCX固相萃取小柱净化后，采用高效液相色谱仪测定。方法的检测限和定量限分别为10、25μg/kg。羊肉、羊肝、羊肾和羊脂肪在25—600μg/kg添加浓度范围内，回收率为74．4％～107％，批内RSD在0．9％～11．9％之间，批间RSD在0．3％～11．1％之间。鸡肉、鸡肝、鸡脂肪和鸭肉在25～600μg/kg添加浓度范围内，回收率为74．9％～101％，批内RSD在1．9％～10．7％之间，批间RSD在1．2％～7．1％之间。结果表明，本方法能满足动物性食品中环丙氨嗪测定的需要。
건립료고효액상색보법측정금、양조직중배병안진잔류량적방법。동물조직양품중잔류적배병안진경삼록을산／을정용액제취，MCX고상췌취소주정화후，채용고효액상색보의측정。방법적검측한화정량한분별위10、25μg/kg。양육、양간、양신화양지방재25—600μg/kg첨가농도범위내，회수솔위74．4％～107％，비내RSD재0．9％～11．9％지간，비간RSD재0．3％～11．1％지간。계육、계간、계지방화압육재25～600μg/kg첨가농도범위내，회수솔위74．9％～101％，비내RSD재1．9％～10．7％지간，비간RSD재1．2％～7．1％지간。결과표명，본방법능만족동물성식품중배병안진측정적수요。
A high - performance liquid chromatography (HPLC) method for the determination of cyromazine in poultry and sheep tissues was optimized and validated. The tissues were extracted by trichloroacetie acid/ acetonitrile, and a solid -phase extraction (MCX) was followed, finally, the eluate was assayed by HPLC. The results showed that the limit of detection (LOD) was 10 Ixg/kg, and the limit of quantitation (LOQ) was 25 ixg/kg. Recoveries of cyromazine in different sheep tissues were between 74.4% and 107% , when its additive concentrations were between 25 and 600 μg/kg, the coefficient of variation (CV) for within - run assays were between 0.9% and 11.9% and CV for between-run assays were between 0.3% and 11.1%. Meanwhile, the recoveries of cyromazine in poultry different tissues were between 74. 9% and 101%, when its additive concentrations were between 25 and 600 Ixg/kg, the CV for within - run assays and between - run assays were between 1.9% and 10.7% and between 1.2% and 7.1% respectively. It was concluded that this method was simple, sensitive and reliable for food safety.