CAJ | 학술논문

为了评估日本血吸虫还原性辅酶I（SjNADH）在小鼠体内诱导的免疫保护效果,应用PCR获得SjNADH基因片段,其开放阅读框为474 bp,编码157个氨基酸,并在大肠杆菌中成功表达,纯化得到该重组蛋白。Western blot结果显示,SjNADH在日本血吸虫童虫和成虫中的表达量稳定,SjNADH融合蛋白也能被免疫血清识别,具有较好的免疫原性。应用重组蛋白免疫小鼠能诱导产生较高的特异性抗体水平,并诱导了20.13%的减虫率和23.06%的肝脏减卵率。结果表明,获得的SjNADH重组蛋白在小鼠体内诱导产生了部分免疫保护,有作为血吸虫疫苗候选抗原分子的潜力。
위료평고일본혈흡충환원성보매I（SjNADH）재소서체내유도적면역보호효과,응용PCR획득SjNADH기인편단,기개방열독광위474 bp,편마157개안기산,병재대장간균중성공표체,순화득도해중조단백。Western blot결과현시,SjNADH재일본혈흡충동충화성충중적표체량은정,SjNADH융합단백야능피면역혈청식별,구유교호적면역원성。응용중조단백면역소서능유도산생교고적특이성항체수평,병유도료20.13%적감충솔화23.06%적간장감란솔。결과표명,획득적SjNADH중조단백재소서체내유도산생료부분면역보호,유작위혈흡충역묘후선항원분자적잠력。
The objectives of the present study were to clone and express the gene encoding NADH I of Schistosoma japonicum and evaluate its immunoprotection activity in mice.Sequence analysis showed that the ORF of SjNADH was 474 bp,encoding 157 amino acids.The recombinant protein was purified from E.coil BL21（DE3） with His·Bind resin,and showed good immunogenicity in mice.The contents of SjNADH in schistosomula and adult worm were similar as determined in Western blot.Mice vaccinated with recombinant protein showed a high level of specific antibody response,worm burden reduction rate of 20.13% and liver egg burden reduction of 23.06%.Results suggested that the recombinant pET28a（＋）-SjNADH protein had the potential as a vaccine candidate against schistosomiasis.