CAJ | 학술논문

目的：探讨继续囊胚培养筛选非优质胚胎中具有发育潜能胚胎的可行性。方法收集生殖中心142例IVF/ICSI治疗周期中受精后第3天（ D3）移植、冷冻保存后剩余的538枚非优质胚胎继续囊胚培养至第6天（D6），观察其囊胚形成情况，并将其按不同卵裂球数和评级分为4组（4细胞Ⅰ、Ⅱ、Ⅲ级胚胎组、5细胞Ⅰ、Ⅱ、Ⅲ级胚胎组、6～9细胞Ⅲ级胚胎组和＞9细胞Ⅰ、Ⅱ、Ⅲ级胚胎组），比较4组间囊胚形成的情况。结果非优质胚胎的囊胚形成率为65．6％，优质囊胚率为51．3％，其中4个组的囊胚形成率分别是55．6％、56．6％、70．4％和66．7％，差异有统计学意义（ P＜0．05）；优质囊胚率分别是8．9％、32．6％、61．0％和63．3％，差异有统计学意义（P＜0．05）。结论继续囊胚培养能有效筛选出非优质胚胎中具有发育潜能的胚胎，提高其利用率。
목적：탐토계속낭배배양사선비우질배태중구유발육잠능배태적가행성。방법수집생식중심142례IVF/ICSI치료주기중수정후제3천（ D3）이식、냉동보존후잉여적538매비우질배태계속낭배배양지제6천（D6），관찰기낭배형성정황，병장기안불동란렬구수화평급분위4조（4세포Ⅰ、Ⅱ、Ⅲ급배태조、5세포Ⅰ、Ⅱ、Ⅲ급배태조、6～9세포Ⅲ급배태조화＞9세포Ⅰ、Ⅱ、Ⅲ급배태조），비교4조간낭배형성적정황。결과비우질배태적낭배형성솔위65．6％，우질낭배솔위51．3％，기중4개조적낭배형성솔분별시55．6％、56．6％、70．4％화66．7％，차이유통계학의의（ P＜0．05）；우질낭배솔분별시8．9％、32．6％、61．0％화63．3％，차이유통계학의의（P＜0．05）。결론계속낭배배양능유효사선출비우질배태중구유발육잠능적배태，제고기이용솔。
Objective To investigate the feasibility of screening viable embryos from poor quality embryos by extending blastocyst culture .Methods 538 D3 poor quality embryos from 142 cases IVF/ICSI treatment were collected after embryo transfer or freezing , and observe the blastocyst formation by blastocyst cultured for up to the sixth days ( D6 ) .The embryos were divided into four groups ( 4 cells gradeⅠ/Ⅱ/Ⅲembryos, 5 cells gradeⅠ/Ⅱ/Ⅲ embryos, 6 ~9 cells grade Ⅲ embryos and exceed 9 cells gradeⅠ/Ⅱ/Ⅲembryos) according to different blastomere and grade , and then compared the blastula development .Results The blastula formation rate and the high-quality blastula rate from D3 poor quality embryos were 65.6%and 51. 3%, the blastula formation rate of the four groups were 55.6%,56.6%,70.4% and 66.7%, there were significant difference among the four groups (P<0.05).And the high-quality blastula rate were 8.9%, 32.6%, 61.0% and 63.3%, there were significant difference among the four groups (P <0.05).Conclusions Extending blastocysts cultured can select the developmental potential embryos from those poor quality embryos remnant effectively , and make better use of the embryos .