CAJ | 학술논문

目的：观察铜负荷饮食对大鼠空间学习记忆行为和海马超微结构的影响。方法40只雄性大鼠随机分为模型组和空白对照组各20只，采用Morris水迷宫实验测试大鼠学习记忆能力，采用原子吸收法检测肝、脑组织铜元素含量，电镜技术观察海马区超微结构变化。结果 Morris水迷宫实验：模型组大鼠在定位航行试验中平均逃避潜伏期较正常对照组显著延长，空间探索试验中穿越平台次数较正常组明显减少（P＜0．01），与正常对照组比较，模型组大鼠肝、脑组织铜含量明显增加（P＜0．01），海马组织内模型组大鼠神经元突触间隙明显变宽（P＜0．05），突触后致密物厚度明显变薄（P＜0．05）。结论铜可能通过损伤海马超微结构引起大鼠学习记忆功能障碍。
목적：관찰동부하음식대대서공간학습기억행위화해마초미결구적영향。방법40지웅성대서수궤분위모형조화공백대조조각20지，채용Morris수미궁실험측시대서학습기억능력，채용원자흡수법검측간、뇌조직동원소함량，전경기술관찰해마구초미결구변화。결과 Morris수미궁실험：모형조대서재정위항행시험중평균도피잠복기교정상대조조현저연장，공간탐색시험중천월평태차수교정상조명현감소（P＜0．01），여정상대조조비교，모형조대서간、뇌조직동함량명현증가（P＜0．01），해마조직내모형조대서신경원돌촉간극명현변관（P＜0．05），돌촉후치밀물후도명현변박（P＜0．05）。결론동가능통과손상해마초미결구인기대서학습기억공능장애。
Objective To determine the effects of copper-overloaded diet on learning and memory and hip-pocampal ultra-microstructure of rats. Methods Forty male SD rats were random assigned to 2 control and model group, respectively. Morris water maze test was used to evaluate the learning and memory capacity. The brain and liv-er were biopsied for the measurement of copper contents. The ultra-microstructure in hippocampus were observed by using transmission electron microscope. Results The mean escape latent period was markedly prolonged and the number of traversing flat roof of the model group significantly decreased compared with those of control group (both P<0.01). The tissue levels of copper were increased in the model group (P<0.01). This was accompanied by the widen-ing of synaptic cleft and reduction in the PSD thickness (both P<0.05) in the model group. Conclusion Copper-overloaded diet can elicit injury of the hippocampus neurons via damage of the ultra-microstructures of the hippocam-pus.