中国调味品
中國調味品
중국조미품
CHINESE CONDIMENT
2014年
11期
120-122,123
,共4页
生物传感器%酶电极%H2 O2%固定化%谷氨酸钠测定
生物傳感器%酶電極%H2 O2%固定化%穀氨痠鈉測定
생물전감기%매전겁%H2 O2%고정화%곡안산납측정
bio-sensor%enzyme electrode%H2 O2%immobilized%determination of sodium glutamate
建立一种新的测定酱油中 L-谷氨酸钠含量的方法———固定化谷氨酸氧化酶电极生物传感分析法。谷氨酸氧化酶专一性地与L-谷氨酸(根)反应,产生的过氧化氢在电极表面上发生电子转移,内置电子元件将电信号转变为数字信号,即可准确、快捷、方便地实现 L-谷氨酸钠的定量。而且,该方法既不需对酱油脱色亦不需进行衍生化,实际测定酱油中L-谷氨酸钠的含量为4700 mg/dL,用氨基酸分析仪检测谷氨酸总含量为6704 mg/dL。酶电极测定的线性范围：0~128 mg/dL,各点测定偏差均小于0.5%,响应时间：20 s。对其进行3次平行测定,实验误差均在±1%之内,另外,酶膜安装在仪器上之后,正常操作能重复测定1000次以上,具有很好的稳定性。
建立一種新的測定醬油中 L-穀氨痠鈉含量的方法———固定化穀氨痠氧化酶電極生物傳感分析法。穀氨痠氧化酶專一性地與L-穀氨痠(根)反應,產生的過氧化氫在電極錶麵上髮生電子轉移,內置電子元件將電信號轉變為數字信號,即可準確、快捷、方便地實現 L-穀氨痠鈉的定量。而且,該方法既不需對醬油脫色亦不需進行衍生化,實際測定醬油中L-穀氨痠鈉的含量為4700 mg/dL,用氨基痠分析儀檢測穀氨痠總含量為6704 mg/dL。酶電極測定的線性範圍：0~128 mg/dL,各點測定偏差均小于0.5%,響應時間：20 s。對其進行3次平行測定,實驗誤差均在±1%之內,另外,酶膜安裝在儀器上之後,正常操作能重複測定1000次以上,具有很好的穩定性。
건립일충신적측정장유중 L-곡안산납함량적방법———고정화곡안산양화매전겁생물전감분석법。곡안산양화매전일성지여L-곡안산(근)반응,산생적과양화경재전겁표면상발생전자전이,내치전자원건장전신호전변위수자신호,즉가준학、쾌첩、방편지실현 L-곡안산납적정량。이차,해방법기불수대장유탈색역불수진행연생화,실제측정장유중L-곡안산납적함량위4700 mg/dL,용안기산분석의검측곡안산총함량위6704 mg/dL。매전겁측정적선성범위：0~128 mg/dL,각점측정편차균소우0.5%,향응시간：20 s。대기진행3차평행측정,실험오차균재±1%지내,령외,매막안장재의기상지후,정상조작능중복측정1000차이상,구유흔호적은정성。
A new method for the determination of L-glutamic acid sodium in soy sauce-immobilized glutamic acid oxidase electrode biology sensing analysis is established.Glutamic acid oxidase specific-ally to react with L-glutamic acid (root),hydrogen peroxide produced undergoes electron transfer re-action on the electrode surface,the built-in electronic components turn electrical signals into digital signals,then the quantitative of L-glutamic acid sodium can be realized accurately,fast and easily. Moreover,this method neither need the decolorization of soy sauce nor derivatization,in the actual de-termination of L-glutamic acid sodium in soy sauce,the content of sodium is 4700 mg/dL,using ami-no acid analyzer to determine the amount of glutamate content is 6704 mg/dL.The linear range of en-zyme electrode determination is 0~128 mg/dL,the measurement deviation of all the points is less than 0.5%,the response time is 20 s.Among the three parallel determination,the experiment error is within ±1%,in addition,the normal operation can repeat determination of more than one thousand times after installation of the enzyme membrane on the instrument,which has a good stability.
