CAJ | 학술논문

采用 Agilent Eclipse XDB-C18色谱柱,以0.02 mol/L 乙酸铵溶液(含0.2%三乙胺,冰醋酸调 pH至4.0)+甲醇为流动相,梯度洗脱,HPLC 法同时测定泡菜中的柠檬黄、胭脂红、日落黄和诱惑红。制备模拟阳性样品并进行方法学考察,以无水乙醇-2%氨水-水(7∶2∶1)为溶剂提取样品中的色素,用聚酰胺除杂质、提纯。结果4种色素质量分别在17.14~342.7 ng,16.67~333.3 ng,15.97~319.3 ng 和16.51~330.1 ng 范围内与峰面积呈良好线性关系,4种色素的加样回收率分别为99.8%,99.8%,99.0%和99.1%,RSD 均低于2.0%。实验结果表明此方法精密度高,重现性好,定量准确,可对泡菜中添加的合成色素进行有效监控。
채용 Agilent Eclipse XDB-C18색보주,이0.02 mol/L 을산안용액(함0.2%삼을알,빙작산조 pH지4.0)+갑순위류동상,제도세탈,HPLC 법동시측정포채중적저몽황、연지홍、일락황화유혹홍。제비모의양성양품병진행방법학고찰,이무수을순-2%안수-수(7∶2∶1)위용제제취양품중적색소,용취선알제잡질、제순。결과4충색소질량분별재17.14~342.7 ng,16.67~333.3 ng,15.97~319.3 ng 화16.51~330.1 ng 범위내여봉면적정량호선성관계,4충색소적가양회수솔분별위99.8%,99.8%,99.0%화99.1%,RSD 균저우2.0%。실험결과표명차방법정밀도고,중현성호,정량준학,가대포채중첨가적합성색소진행유효감공。
The Agilent Eclipse XDB-C1 8 column is used,with 0.02 mol/L ammonium acetate solution (containing 0.2% triethylamine,adjusted to pH 4.0 )and methanol as the mobile phase,gradient eluting.The HPLC method is applied to determine the tartrazine,carmine,sunset yellow and allura red in pickled vegetables.Simulated positive samples are prepared and methodology investigating is implemented.Anhydrous ethanol-2% ammonia-water (7 ∶ 2 ∶ 1 )is used as the extracting agent to extract the pigment in samples,then the polyamide is used in impurity removal and purification.The mass of four pigments shows good linear relation with the peak area in the scope of 17.14~342.7 ng, 1 6.67~333.3 ng,15.97~31 9.3 ng and 1 6.5 1~330.1 ng.The percent recoveries of the pigments are 99.8%,99.8%,99.0%and 99.1%,RSDs are all below 2%.The experimental results show that this method is precise,reproducible,accurate,and could effectively monitor the pigments added in pickled vegetables.