CAJ | 학술논문

通过检测生姜提取物对邻苯三酚自氧化反应生成的超氧自由基的清除,表征其抗氧化能力。首先确定邻苯三酚自氧化反应条件,然后加入多种生姜提取物,检测其对超氧自由基的清除效果。检测方法为测反应产生的半醌在320 nm 处的紫外吸光度。结果表明邻苯三酚自氧化反应条件为：反应温度24℃,体系 pH 8.40~8.52,反应时间17~18 min。各类提取物中,以姜酚为主的粗分物对超氧自由基的清除率最高,清除率与提取物浓度呈线性关系。相同浓度下,超临界 CO2萃取分离物对超氧自由基的清除率明显高于相应的溶剂萃取分离物。
통과검측생강제취물대린분삼분자양화반응생성적초양자유기적청제,표정기항양화능력。수선학정린분삼분자양화반응조건,연후가입다충생강제취물,검측기대초양자유기적청제효과。검측방법위측반응산생적반곤재320 nm 처적자외흡광도。결과표명린분삼분자양화반응조건위：반응온도24℃,체계 pH 8.40~8.52,반응시간17~18 min。각류제취물중,이강분위주적조분물대초양자유기적청제솔최고,청제솔여제취물농도정선성관계。상동농도하,초림계 CO2췌취분리물대초양자유기적청제솔명현고우상응적용제췌취분리물。
The antioxidant capacity of ginger active ingredients is characterized by the ability of scavenging superoxide radical,which is produced by pyrogallol auto-oxidation action.The experiment factors of pyrogallol auto-oxidation action including temperature,reaction time,and pH are firstly researched.Then the ginger extracts are added into the mixture to scavenge the superoxide radical. The detection method is measuring the UV absorbance value of semiquinone at 320 nm.The results show that the appropriate conditions are:24 ℃,17~18 min,and pH 8.40~8.52.The samples with gingerol as the main ingredient have the highest scavenging ratio of superoxide radical,and the scavenging ratio has a linear relationship with the extraction concentration.The scavenging ratio of supercritical CO2 extraction is obviously higher than solvent extraction under the same mass concentration.