广西植物
廣西植物
엄서식물
GUIHAIA
2014年
2期
183-188
,共6页
颜小捷%刘幼娴%郑立浪%卢凤来%李典鹏
顏小捷%劉幼嫻%鄭立浪%盧鳳來%李典鵬
안소첩%류유한%정립랑%로봉래%리전붕
核桃蛋白%胃蛋白酶%木瓜蛋白酶%水解度
覈桃蛋白%胃蛋白酶%木瓜蛋白酶%水解度
핵도단백%위단백매%목과단백매%수해도
walnut protein%epsin%papain%degree of hydrolysis
以核桃粕为原料,以水解度为考察指标,研究胃蛋白酶和木瓜蛋白酶对核桃蛋白的水解作用.结果表明:木瓜蛋白酶为酶解核桃蛋白最佳酶,其最佳酶解条件为底物浓度4%、酶质量分数5%、酶解 pH 值为7.0、酶解温度50℃、酶解时间4 h;在该条件下,木瓜蛋白酶酶解核桃蛋白水解度可高于25.76%.
以覈桃粕為原料,以水解度為攷察指標,研究胃蛋白酶和木瓜蛋白酶對覈桃蛋白的水解作用.結果錶明:木瓜蛋白酶為酶解覈桃蛋白最佳酶,其最佳酶解條件為底物濃度4%、酶質量分數5%、酶解 pH 值為7.0、酶解溫度50℃、酶解時間4 h;在該條件下,木瓜蛋白酶酶解覈桃蛋白水解度可高于25.76%.
이핵도박위원료,이수해도위고찰지표,연구위단백매화목과단백매대핵도단백적수해작용.결과표명:목과단백매위매해핵도단백최가매,기최가매해조건위저물농도4%、매질량분수5%、매해 pH 치위7.0、매해온도50℃、매해시간4 h;재해조건하,목과단백매매해핵도단백수해도가고우25.76%.
Walnut peptides were obtained through pepsin or papain enzymolysis with walnut residue as raw materials and degree of hydrolysis as index.The results showed that papain was the optimum proteinase.The optimum hydro-lytic conditions were as followed:substrate concentration was 4%,enzyme concentration was 5%,pH was 7,tempera-ture was 50 ℃ and hydrolysis time was 4 h.Under these optimum conditions,the degree of hydrolysis can be higher than 25.76%.