CAJ | 학술논문

采用高效液相色谱法，测定金钱菇多糖的单糖组成.用超声辅助提取金钱菇多糖，通过1-苯基-3-甲基-5-吡唑酮(PMP)衍生水解后的单糖，高效液相色谱法检测衍生物.结果表明：金钱菇多糖由甘露糖(Man)、核糖(Rib)、鼠李糖(Rha)、葡萄糖(Glc)、半乳糖(Gal)、木糖(Xyl)组成，其摩尔为1．00∶0．90∶0．91∶28．03∶1．58∶0．11.该方法快速、简便、重现性好，可用于测定金钱菇多糖的单糖组成.
채용고효액상색보법，측정금전고다당적단당조성.용초성보조제취금전고다당，통과1-분기-3-갑기-5-필서동(PMP)연생수해후적단당，고효액상색보법검측연생물.결과표명：금전고다당유감로당(Man)、핵당(Rib)、서리당(Rha)、포도당(Glc)、반유당(Gal)、목당(Xyl)조성，기마이위1．00∶0．90∶0．91∶28．03∶1．58∶0．11.해방법쾌속、간편、중현성호，가용우측정금전고다당적단당조성.
Monosaccharide composition in Lentinus edode polysaccharides was determined by high performance liquid chromatography(HPLC).The L .edodes polysaccharide was extracted using ultrasonic-assisted extraction.Monosac-charide were derived by 1-phenyl-3-methyl-5-pyrazolone(PMP)and the PMP derivatives of monosaccharide were an-alyzed by HPLC.The results showed that L .edodes polysaccharide was composed of mannose,ribose,rhamnose,glu-cose,galactose and xylose and their molar ratio was about 1.00∶0.90∶0.91∶28.03∶1.58∶0.11.The method was rapid,simple and reproducible and could be used to determine monosaccharide compositions of L .edodes .