广西植物
廣西植物
엄서식물
GUIHAIA
2014年
2期
174-178
,共5页
刘幼娴%谷陟欣%卢凤来%黄胜%颜小捷%李典鹏
劉幼嫻%穀陟訢%盧鳳來%黃勝%顏小捷%李典鵬
류유한%곡척흔%로봉래%황성%안소첩%리전붕
裸花紫珠%高效液相色谱法%指纹图谱%主成分分析%对应分析
裸花紫珠%高效液相色譜法%指紋圖譜%主成分分析%對應分析
라화자주%고효액상색보법%지문도보%주성분분석%대응분석
Callicarpa nudiflora%high performance liquid chromatographic%fingerprint%hierarchical clustering anal-ysis%correspondence analysis
为建立裸花紫珠药材高效液相指纹图谱研究方法,该研究采用0.4%磷酸水溶液(A)-乙腈(B);梯度洗脱为0~15 min,16%~25% B,15~25 min,25%~39.7% B;Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm);柱温为30℃;流速为1 mL??min-1;进样量为10μL;检测波长327 nm;检测时间25 min 等色谱条件,研究了不同采集时间共25批裸花紫珠药材的指纹图谱,获取14个共有特征色谱峰,确定了其中的3个成分,利用相似度分析、主成分分析及对应分析对它们进行了分析.结果表明:HPLC 指纹图谱结合计量分析法可有效评价裸花紫珠的质量,该方法可有效应用到裸花紫珠原料及其制品的质量控制中.
為建立裸花紫珠藥材高效液相指紋圖譜研究方法,該研究採用0.4%燐痠水溶液(A)-乙腈(B);梯度洗脫為0~15 min,16%~25% B,15~25 min,25%~39.7% B;Agilent ZORBAX SB-C18色譜柱(4.6 mm×250 mm,5μm);柱溫為30℃;流速為1 mL??min-1;進樣量為10μL;檢測波長327 nm;檢測時間25 min 等色譜條件,研究瞭不同採集時間共25批裸花紫珠藥材的指紋圖譜,穫取14箇共有特徵色譜峰,確定瞭其中的3箇成分,利用相似度分析、主成分分析及對應分析對它們進行瞭分析.結果錶明:HPLC 指紋圖譜結閤計量分析法可有效評價裸花紫珠的質量,該方法可有效應用到裸花紫珠原料及其製品的質量控製中.
위건립라화자주약재고효액상지문도보연구방법,해연구채용0.4%린산수용액(A)-을정(B);제도세탈위0~15 min,16%~25% B,15~25 min,25%~39.7% B;Agilent ZORBAX SB-C18색보주(4.6 mm×250 mm,5μm);주온위30℃;류속위1 mL??min-1;진양량위10μL;검측파장327 nm;검측시간25 min 등색보조건,연구료불동채집시간공25비라화자주약재적지문도보,획취14개공유특정색보봉,학정료기중적3개성분,이용상사도분석、주성분분석급대응분석대타문진행료분석.결과표명:HPLC 지문도보결합계량분석법가유효평개라화자주적질량,해방법가유효응용도라화자주원료급기제품적질량공제중.
The chromatographic fingerprint was established for evaluating and controlling the quality of Callicarpa nudiflora .The chromatographic analysis was carried out on a ZORBAX SB-C18 column(250 mm × 4.5 mm,5μm).The mobile phase was 0.4% phosphoric acid(A)and acetonitrile(B)with gradient elution(0-15 min,16% B-25% B;15-25 min,25% B-39.7% B).The flow rate was maintained at 1.0 mL??min-1 .The detection wave length was set at 327 nm and the column temperature was controlled at 30 ℃.The sample injection volume was 10 μL.25 different original samples were analyzed,and the chromatographic fingerprints of C .nudiflora were constructed with 14 fingerprint peaks,and 3 main compounds were identified.The Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(Version2004A),hierarchical clustering analysis(HCA)and correspon-dence analysis were applied to classify the samples according to their sources,and satisfactory results were obtained.The chromatographic fingerprint can be used to differentiate the raw materials from different sources and be served as a powerful tool for the further quality control of C .nudiflora .