CAJ | 학술논문

目的：观察齐齐哈尔地区原发性高血压（ EH）患者ACE I/D和CYP11B2-344 C/T基因多态性及其在EH发病中的相互作用。方法 EH患者486例，血压正常者457例。取外周血提取基因组DNA，用PCR-RLFP对ACE I/D和CYP11B2-344 C/T进行基因分型。结果（1）高血压组和血压正常组ACE I/D各种基因型分别为II 0．344/0．326、ID 0．372/0．497、DD 0．284/0．177（P＜0．001），D等位基因频率为0．470/0．404（P ＜0．05）；（2）高血压组和血压正常组 CYP11B2-344 C/T 各种基因型分别为 CC 0．136/0．166、CT 0．469/0．438、TT 0．395/0．385（P＞0．05），T等位基因频率为0．630/0．615（P＞0．05）；（3）高血压组DD＋CT联合基因型频率高于血压正常组（P＜0．01）；而高血压组ID＋TT联合基因型频率低于血压正常组（ P ＜0．05）。结论 ACE I/D 多态性与齐齐哈尔地区人群的原发性高血压相关， CYP11B2-344 C/T基因多态性与齐齐哈尔地区人群的原发性高血压不相关；但两基因可能存在相互作用。
목적：관찰제제합이지구원발성고혈압（ EH）환자ACE I/D화CYP11B2-344 C/T기인다태성급기재EH발병중적상호작용。방법 EH환자486례，혈압정상자457례。취외주혈제취기인조DNA，용PCR-RLFP대ACE I/D화CYP11B2-344 C/T진행기인분형。결과（1）고혈압조화혈압정상조ACE I/D각충기인형분별위II 0．344/0．326、ID 0．372/0．497、DD 0．284/0．177（P＜0．001），D등위기인빈솔위0．470/0．404（P ＜0．05）；（2）고혈압조화혈압정상조 CYP11B2-344 C/T 각충기인형분별위 CC 0．136/0．166、CT 0．469/0．438、TT 0．395/0．385（P＞0．05），T등위기인빈솔위0．630/0．615（P＞0．05）；（3）고혈압조DD＋CT연합기인형빈솔고우혈압정상조（P＜0．01）；이고혈압조ID＋TT연합기인형빈솔저우혈압정상조（ P ＜0．05）。결론 ACE I/D 다태성여제제합이지구인군적원발성고혈압상관， CYP11B2-344 C/T기인다태성여제제합이지구인군적원발성고혈압불상관；단량기인가능존재상호작용。
Objective To explore the polymorphism of ACE I/D and CYP11B2-344 C/T and the genetic interaction between ACE and CYP11B2 genes in Qiqhar essential hypetension (EH) Han population.Methods Four hundreds and eighty-six patients with EH and four hundreds and fifty-seven healthy controls were enrolled . Genotyping was performed using PCR-RFLP technique .Results ( 1 ) genotype distributions of ACE I/D ( II 0.344 vs 0.326, ID 0.372 vs 0.497, DD 0.284 vs 0.177,P<0.001) between EH group and NT group;there was significant differences in D allele frequency of ACE I /D (0.470 vs 0.404, P<0.05) between two group. (2) genotype distributions of CYP11B2-344 C/T ( CC 0.136 vs 0.166, CT 0.469 vs 0.438, TT 0.395 vs 0.385, P>0.05) between EH group and NT group;there was not significant differences in T allele frequency of CYP11B2-344 C/T (0.630 vs 0.615, P>0.05) between two group.(3) The genotype distributions of DD +CT of EH group was significantly higher than that of NT group (P<0.01), the genotypes distribution of ID +TT of EH group was significantly lower than that of NT group (P<0.05).Conclusions These observations suggest that ACE I/D polymorphism is associated with essential hypertension in Qiqihar Han population , CYP11B2-344 C/T polymorphism is not associated with essential hypertension in Qiqihar Han population , and there might be mutual effect between the two genes .