CAJ | 학술논문

目的：研究鹰嘴豆芽素 A （ BCA ）及其聚乙二醇化纳米脂质载体（ PEG-BCA-NLC）的细胞吸收转运机制。方法用Caco-2细胞模型研究BCA及PEG-BCA-NLC的摄取、转运及其影响因素；用高效液相色谱法检测BCA浓度，计算其表观通透系数（ Papp ）。结果 Caco-2细胞对BCA及PEG-BCA-NLC的吸收符合被动扩散过程，但相同时间下BCA的摄取量较PEG-BCA-NLC高；加入维拉帕米可有效提高Caco-2细胞对PEG-BCA-NLC的吸收转运，Papp （肠腔侧→基底层，AP→BL）增大，而Papp（ BL→AP）变小，但转运量仍较BCA溶液组低。结论 PEG-BCA-NLC在Caco-2细胞模型中的吸收转运主要是被动扩散方式，PEG-BCA-NLC不一定能显著提高药物在小肠的吸收。
목적：연구응취두아소 A （ BCA ）급기취을이순화납미지질재체（ PEG-BCA-NLC）적세포흡수전운궤제。방법용Caco-2세포모형연구BCA급PEG-BCA-NLC적섭취、전운급기영향인소；용고효액상색보법검측BCA농도，계산기표관통투계수（ Papp ）。결과 Caco-2세포대BCA급PEG-BCA-NLC적흡수부합피동확산과정，단상동시간하BCA적섭취량교PEG-BCA-NLC고；가입유랍파미가유효제고Caco-2세포대PEG-BCA-NLC적흡수전운，Papp （장강측→기저층，AP→BL）증대，이Papp（ BL→AP）변소，단전운량잉교BCA용액조저。결론 PEG-BCA-NLC재Caco-2세포모형중적흡수전운주요시피동확산방식，PEG-BCA-NLC불일정능현저제고약물재소장적흡수。
Objective To investigate the absorption mechanism of Bio-chanin A ( BCA) and PEGylated BCA loaded nanostructured lipid carri-ers ( PEG -BCA -NLC ) across Caco -2 cell model.Methods Caco-2 cell monolayer model was used to explore the uptake , transport and impact factors of BCA and PEG -BCA-NLC.Drug concentration was measured by HPLC and the apparent permeability coefficients ( Papp ) were calculated.Results The main mechanism of BCA and PEG-BCA-NLC across Caco -2 monolayer model was passive diffu-sion.But under the same time , lower uptake of drug in Caco -2 cells was observed at PEG -BCA -NLC group comparing to that at BCA group.The absorption and the apparent permeability coefficient of BCA in Caco-2 cells monolayer were increased when combined with verapam-il.The Papp (AP→BL) increased and Papp (BL→PL) reduced, but the uptake content was much lower than BCA solution.Conclusion Ab-sorption process of PEG -BCA-NLC in Caco-2 cells monolayer met the characteristics of passive diffusion.However , the PEG-BCA-NLC may not significantly improve the absorption of BCA in intestinal epithelial.