CAJ | 학술논문

目的：探讨心理应激状态下大鼠咬肌异常活动度与颞下颌关节病变程度之间的相关性。方法选取36只成年Wistar大鼠，随机分为3组，即实验3周组、实验6周组和空白对照组，每组12只。对实验3周组和实验6周组分别施加预定刺激3周和6周，使大鼠处于应激状态。处死3组动物，分别取大鼠颞下颌关节和咬肌组织，制作关节病理切片，观察关节组织结构病理变化；定量咬肌组织肌肉匀浆检测乳酸脱氢酶（LDH）和肌酸激酶（CK）含量，并分析每份标本中咬肌LDH、CK含量与颞下颌关节病变程度之间的相关性。结果应激状态下大鼠咬肌肌肉中LDH、CK含量与颞下颌关节病变程度呈正相关；应激状态下大鼠咬肌肌肉中LDH、CK含量影响颞下颌关节病变程度。结论应激状态下咬肌异常活动度有可能作为大鼠颞下颌关节病理变化程度的参考指标，咬肌异常活动可能是致颞下颌关节病变的途径之一。
목적：탐토심리응격상태하대서교기이상활동도여섭하합관절병변정도지간적상관성。방법선취36지성년Wistar대서，수궤분위3조，즉실험3주조、실험6주조화공백대조조，매조12지。대실험3주조화실험6주조분별시가예정자격3주화6주，사대서처우응격상태。처사3조동물，분별취대서섭하합관절화교기조직，제작관절병리절편，관찰관절조직결구병리변화；정량교기조직기육균장검측유산탈경매（LDH）화기산격매（CK）함량，병분석매빈표본중교기LDH、CK함량여섭하합관절병변정도지간적상관성。결과응격상태하대서교기기육중LDH、CK함량여섭하합관절병변정도정정상관；응격상태하대서교기기육중LDH、CK함량영향섭하합관절병변정도。결론응격상태하교기이상활동도유가능작위대서섭하합관절병리변화정도적삼고지표，교기이상활동가능시치섭하합관절병변적도경지일。
Objective??This?study?aims?to?investigate?the?correlation?between?the?rat?masseter?abnormal?activity?and?the?degree?of?pathological?changes?of?temporomandibular?joint?disease?under?mental?stress.?Methods???A?total?of?36?adult?Wistar?rats?were?randomly?divided?into?three?groups:?three-week?experimental,?six-week?experimental,?and?control?groups(n=12?for?each?group).?A?predetermined?stimulus?was?applied?on?the?experimental?groups?to?place?the?rats?in?a?stress?state.?The?three?rat?groups?were?then?sacrificed.?The?rat?temporomandibular?joint?and?masseter?muscle?tissue?were?obtained?and?submitted?to?the?joint?pathological?section?for?observation?of?the?pathological?changes?of?the?joint?organizational?structure.?The?levels?of?lactate?dehydrogenase(LDH)?and?creatine?kinase(CK)?were?quantitatively?determined?in?the?masseter?muscle?tissue?homogenates.??Spearman?correlation?and?multiple?regression?analyses?were?used?for?statistical?analysis.?Analysis?of?the?CK?and?LDH?contents?in?each?specimen?masseter?muscle?was?carried?out?to?determine?their?correlation?to?the?severity?of?the?temporomandibular?joint.?Results???A?positive?correlation?was?observed?between?the?LDH?and?CK?contents?in?the?masseter?muscle?of?rats?to?the?severity?of?the?temporomandibular?joint.?The?levels?of?LDH?and?CK?in?the?masseters?of?stressed?rats?showed?a?reciprocal?relationship?to?the?extent?of?temporomandibular?joint?disease.?Conclusion??The?masseter?muscle?abnormal?activity?under?stress?may?be?used?as?a?reference?indicator?of?the?degree?of?pathological?changes?of?the?rat?temporomandibular?joint.?The?masseter?muscle?abnormal?activity?is?possibly?caused?by?a?temporomandibular?joint?disease.