华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2014年
3期
297-302
,共6页
鄢国伟%黄文明%薛红蕾%贾玉焕%杨德琴
鄢國偉%黃文明%薛紅蕾%賈玉煥%楊德琴
언국위%황문명%설홍뢰%가옥환%양덕금
龋病%唾液蛋白组%电喷雾离子肼-串联质谱%生物标志物
齲病%唾液蛋白組%電噴霧離子肼-串聯質譜%生物標誌物
우병%타액단백조%전분무리자정-천련질보%생물표지물
dental caries%salivary proteome%elec-trospray ionization ion-trap tandem mass spectrometry%biomarkers
目的:采用电喷雾离子肼-串联质谱分析法(ESI-MS/MS)比较高龋组和无龋组儿童唾液蛋白组,初步探索唾液蛋白组与龋病的关系,并探索龋敏感性相关的生物标志物。方法以高龋组、无龋组儿童各10名为研究对象,收集唾液样本,进行聚丙烯酰胺凝胶电泳(SDS-PAGE),经过滤-辅助的蛋白酶解(FASP)及液相柱色谱分析后,进行ESI-MS/MS鉴定,并对高龋唾液组和无龋唾液组组间的蛋白差异进行分析。结果高龋组蛋白量显著高于无龋组,高龋组、无龋组鉴定的多肽数分别为602、481个,分别属于286、227个蛋白。两组之间差异表达多肽数为361个,差异表达蛋白数为118个,包含基质金属蛋白酶9、黏蛋白7、乳铁蛋白、碳酸酐酶6、天青杀素、冷凝集素等。结论高龋组儿童唾液蛋白量高于无龋组,基质金属蛋白酶9、黏蛋白7、乳铁蛋白、碳酸酐酶6、天青杀素、冷凝集素等高龋组与无龋组之间差异表达蛋白的检测,为进一步探索龋敏感性相关生物蛋白标志物奠定了基础。
目的:採用電噴霧離子肼-串聯質譜分析法(ESI-MS/MS)比較高齲組和無齲組兒童唾液蛋白組,初步探索唾液蛋白組與齲病的關繫,併探索齲敏感性相關的生物標誌物。方法以高齲組、無齲組兒童各10名為研究對象,收集唾液樣本,進行聚丙烯酰胺凝膠電泳(SDS-PAGE),經過濾-輔助的蛋白酶解(FASP)及液相柱色譜分析後,進行ESI-MS/MS鑒定,併對高齲唾液組和無齲唾液組組間的蛋白差異進行分析。結果高齲組蛋白量顯著高于無齲組,高齲組、無齲組鑒定的多肽數分彆為602、481箇,分彆屬于286、227箇蛋白。兩組之間差異錶達多肽數為361箇,差異錶達蛋白數為118箇,包含基質金屬蛋白酶9、黏蛋白7、乳鐵蛋白、碳痠酐酶6、天青殺素、冷凝集素等。結論高齲組兒童唾液蛋白量高于無齲組,基質金屬蛋白酶9、黏蛋白7、乳鐵蛋白、碳痠酐酶6、天青殺素、冷凝集素等高齲組與無齲組之間差異錶達蛋白的檢測,為進一步探索齲敏感性相關生物蛋白標誌物奠定瞭基礎。
목적:채용전분무리자정-천련질보분석법(ESI-MS/MS)비교고우조화무우조인동타액단백조,초보탐색타액단백조여우병적관계,병탐색우민감성상관적생물표지물。방법이고우조、무우조인동각10명위연구대상,수집타액양본,진행취병희선알응효전영(SDS-PAGE),경과려-보조적단백매해(FASP)급액상주색보분석후,진행ESI-MS/MS감정,병대고우타액조화무우타액조조간적단백차이진행분석。결과고우조단백량현저고우무우조,고우조、무우조감정적다태수분별위602、481개,분별속우286、227개단백。량조지간차이표체다태수위361개,차이표체단백수위118개,포함기질금속단백매9、점단백7、유철단백、탄산항매6、천청살소、냉응집소등。결론고우조인동타액단백량고우무우조,기질금속단백매9、점단백7、유철단백、탄산항매6、천청살소、냉응집소등고우조여무우조지간차이표체단백적검측,위진일보탐색우민감성상관생물단백표지물전정료기출。
Objective To explore the relationship between salivary proteome and dental caries and to promote the bio-marker studies of dental caries susceptibility by comparing the salivary proteome of caries-active children and caries-free children with electrospray ionization ion-trap tandem mass spectrometry (ESI-MS/MS). Methods Ten caries-active children and ten caries-free children were sampled. The salivary proteome of the two groups was studied, and the differential protein between the two groups was analyzed by ESI-MS/MS after sodium dodecyl sulfate polyacrylamide gel electrophoresis, filter-aided sample preparation, and liquid chromatography. Results The concentration of salivary protein was higher in the caries-active group than in the caries-free group. The polypeptide counts of thecaries-active and caries-free groups were 602 and 481, which belonged to 286 and 227 proteins, respectively. The differential polypeptide count of the two groups was 361, and the differential protein count was 118. The detected proteins included matrix metalloproteinase-9 (MMP9), mucin-7 (MUC7), lactotransferrin (LTF), carbonic anhydrase 6 (CA6), azurocidin (AZU), and cold agglutinin. Conclusion The total salivary protein was higher in the caries-active group than in the caries-free group. The preliminary detection of differential proteins (MMP9, MUC7, LTF, CA6, AZU, and cold agglutinin) may lay some foundation for biomarker research of dental caries susceptibility.