CAJ | 학술논문

目的：初步分析纳米表面修饰的钛棒与兔模型骨界面的愈合情况，探讨钛基纳米棒的成骨生物学性能。方法场发射扫描电镜（FESEM）观察纳米钛棒的表面形态，能量分散谱（EDS）分析样品表面的元素组成。在新西兰大白兔左右侧胫骨近端随机植入钛棒和纳米钛棒2种材料，分别于术后4、12周取材后进行Micro-CT影像学评估和力学测试，对比分析2种材料骨-材料界面结合强度及破坏载荷的差异。结果FESEM证实纳米棒结构（100nm Ti），EDS分析显示纳米钛棒表面由100%Ti元素组成。材料植入区域未见组织坏死、化脓，植入材料移位、脱落等现象。Micro-CT影像学结果提示，100 nm Ti植入4、12周后骨-材料界面结合强度均较Ti组优异；生物力学测试结果表明，材料植入4、12周100nm Ti表面的破坏载荷均明显高于Ti组，两组比较，差异有统计学意义（P＜0.05）。结论100 nm Ti可提高兔模型骨-材料界面的结合强度，促进骨愈合率。
목적：초보분석납미표면수식적태봉여토모형골계면적유합정황，탐토태기납미봉적성골생물학성능。방법장발사소묘전경（FESEM）관찰납미태봉적표면형태，능량분산보（EDS）분석양품표면적원소조성。재신서란대백토좌우측경골근단수궤식입태봉화납미태봉2충재료，분별우술후4、12주취재후진행Micro-CT영상학평고화역학측시，대비분석2충재료골-재료계면결합강도급파배재하적차이。결과FESEM증실납미봉결구（100nm Ti），EDS분석현시납미태봉표면유100%Ti원소조성。재료식입구역미견조직배사、화농，식입재료이위、탈락등현상。Micro-CT영상학결과제시，100 nm Ti식입4、12주후골-재료계면결합강도균교Ti조우이；생물역학측시결과표명，재료식입4、12주100nm Ti표면적파배재하균명현고우Ti조，량조비교，차이유통계학의의（P＜0.05）。결론100 nm Ti가제고토모형골-재료계면적결합강도，촉진골유합솔。
Objective To analyze the osseointegration at Ti nanorod material-bone interface, and to explore osteogenesis biological effect of nanostructure Ti rod. Methods Field emission scanning electron microscopy (FESEM) was employed to observe the morphology of Ti nanorod surface, energy dispersive spectroscopy (EDS) was applied to analyze the elements of the materials. Ti rod and Ti nanorod samples were randomly implanted into either side of tibia of New Zealand white rabbits. After 4, 12 weeks, Micro-CT and push-out test were applied to compare the differences of osseointegration at the material-tibia interface as well as damage load between 2 materials. Results FESEM showed 100 nm Ti surface were acquired and EDS showed nanorod were composed of Ti element only. No tissue necrosis or wound infection were observed and no loosening or pull-out of implants had happened. Four weeks and 12 weeks after implanting, Micro-CT showed osseointegration density in 100 nm Ti group was better than that in Ti group; damage load in 100 nm Ti group was also higher than the result in Ti group, there was statistical difference between two groups (P <0.05). Conclusion 100 nm Ti could improve the osseointegration density at the bone-material interface, and subsequently enhance bone healing rate in rabbit models.