CAJ | 학술논문

目的：探讨组织化学染色技术是否可以应用于塑化标本并验证染色塑化标本是否具有自发荧光。方法选取1个手掌标本经超薄生物塑化技术做成组织块，进行连续切片，切片数量56张，并按切片顺序进行染色处理：原始切片，苏木素-伊红染色，凡尔霍夫-酸性品红染色，亚甲蓝-天青Ⅱ号染色，在扫描仪、光学显微镜和激光扫描共焦显微镜下观察染色效果和组织结构特点并进行比较。结果3种染色技术都可应用于塑化切片染色。苏木素-伊红染色显示肌肉、结缔组织呈红色或紫红色，骨质呈紫蓝色或蓝色。凡尔霍夫-酸性品红染色显示弹力纤维呈黑色，胶原呈红色，其他组织为黄色或棕黄色。亚甲蓝-天青Ⅱ号染色显示肌腱呈紫红色，骨质呈粉红色，软骨呈紫罗兰，其他组织呈紫色。但细胞内结构的染色效果并不理想。在激光扫描共焦显微镜下，胶原纤维、弹力纤维和肌肉纤维具有自发荧光，结构清晰可辨。结论常用的组织化学染色技术适合于超薄塑化切片染色，染色切片的各种组织结构比未染色的观察效果好。染色后，塑化切片中具有自发荧光的结构在激光扫描共焦显微镜下亦可清晰显影。
목적：탐토조직화학염색기술시부가이응용우소화표본병험증염색소화표본시부구유자발형광。방법선취1개수장표본경초박생물소화기술주성조직괴，진행련속절편，절편수량56장，병안절편순서진행염색처리：원시절편，소목소-이홍염색，범이곽부-산성품홍염색，아갑람-천청Ⅱ호염색，재소묘의、광학현미경화격광소묘공초현미경하관찰염색효과화조직결구특점병진행비교。결과3충염색기술도가응용우소화절편염색。소목소-이홍염색현시기육、결체조직정홍색혹자홍색，골질정자람색혹람색。범이곽부-산성품홍염색현시탄력섬유정흑색，효원정홍색，기타조직위황색혹종황색。아갑람-천청Ⅱ호염색현시기건정자홍색，골질정분홍색，연골정자라란，기타조직정자색。단세포내결구적염색효과병불이상。재격광소묘공초현미경하，효원섬유、탄력섬유화기육섬유구유자발형광，결구청석가변。결론상용적조직화학염색기술괄합우초박소화절편염색，염색절편적각충조직결구비미염색적관찰효과호。염색후，소화절편중구유자발형광적결구재격광소묘공초현미경하역가청석현영。
Objective To investigate whether and what staining techniques are applied to the ultrathin sheet plastination slice and whether the stained specimen is of autofluorescences .Methods A cadaveric hand block was plastinated and then sectioned as a series of 300-400μm thick transverse sections .A total of 56 slices in total .Alternative sections were stained with hematoxylin -eosin staining ( HE) , Verhoeff -Van Gieson staining ( VVG) or methylene blue and azureⅡstaining(MA).The stained slices were examined under a light microscope and a confocal microscope .Results The plastinated slices were stained with the three staining methods .HE staining revealed the muscle and connective tissues were red or violet , bone was violet or blue;VVG staining showed the elastic fibers was black , the collagen was red , and other tissues were yellow .MA staining showed the tendon was violet , the bone was pink , cartilage was violet , and other tissues were purple.However, the intracellular structures appeared not very well stained .The collagen, elastin and muscular structures in the stained slices were observed under a confocal microscope .Conclusion The commonly used histology staining methods can be used to stain the ultrathin sheet plastination slices .The staining provides a better observation of various tissues in the slice than the unstained slice .After staining, those autofluorescent structures in the plastinated slice are detectable under a confocal microscope .