CAJ | 학술논문

目的：探讨麻黄素对雌鼠血液雌二醇（ E2）、肾上腺素（ EPI）浓度和卵巢组织结构及超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量的影响。方法受孕雌鼠分别腹腔注射（2g/L、4g/L、6g/L）麻黄素溶液和等量的生理盐水，采用ELISA法检测15d、20d、25d雌鼠血浆中EPI与E2浓度的变化，用比色法检测卵巢SOD活性及MDA含量的变化，称量检测卵巢的重量变化，用生物显微技术观察卵巢组织结构的变化，统计卵巢各级卵泡的数量。结果实验组雌鼠血浆中的E2含量降低，EPI含量显著升高，并呈现剂量依赖性，卵巢SOD活性降低，MDA含量显著升高；实验组卵巢的重量明显低于对照组，初级卵泡、次级卵泡和闭锁卵泡数量均增多；黄体数量减少；实验组卵巢固缩，结构不完整，卵母细胞萎缩，颗粒细胞变性、降解，排列疏松、紊乱，黄体结构模糊，颗粒黄体细胞核固缩。结论麻黄素影响雌鼠卵巢的组织结构和功能，这可能与卵巢中SOD活性下降有关。
목적：탐토마황소대자서혈액자이순（ E2）、신상선소（ EPI）농도화란소조직결구급초양화물기화매（SOD）활성、병이철（MDA）함량적영향。방법수잉자서분별복강주사（2g/L、4g/L、6g/L）마황소용액화등량적생리염수，채용ELISA법검측15d、20d、25d자서혈장중EPI여E2농도적변화，용비색법검측란소SOD활성급MDA함량적변화，칭량검측란소적중량변화，용생물현미기술관찰란소조직결구적변화，통계란소각급란포적수량。결과실험조자서혈장중적E2함량강저，EPI함량현저승고，병정현제량의뢰성，란소SOD활성강저，MDA함량현저승고；실험조란소적중량명현저우대조조，초급란포、차급란포화폐쇄란포수량균증다；황체수량감소；실험조란소고축，결구불완정，란모세포위축，과립세포변성、강해，배렬소송、문란，황체결구모호，과립황체세포핵고축。결론마황소영향자서란소적조직결구화공능，저가능여란소중SOD활성하강유관。
Objective To explore effects of ephedrine on epinephrine (EPI) and estradiol( E2)concentration in blood, histological structures and activity of superoxide dismutase (SOD) and maleic dialdehyde (MDA) content in the ovary of female mice.Methods Pregnant female mice in the experimental group were treated with ephedrine (2g/L,4g/L, 6g/L), and the mice in the control group were treated with saline by intraperitoneal injection .Using ELISA method to detect concentration changes of EPI and E 2 in plasma, the changes in the enzymatic activity of SOD and MDA content were analyzed by colorimetry .The ovarian weight was measured , histological structures in ovary were observed by microscopy and the number of ovarian follicles were counted .Results EPI concentration in plasma of female mice increased , and the E2 concentration decreased dose-dependently in the experimental group .The activities of SOD were lower , and MDA content significantly increased compared to the control .The ovary weight of experimental groups was lower than the control group.The numbers of primary follicle , secondary follicle and atretic follicle increased and corpus luteum decreased in the experimental groups .There were pyknotic ovarian tissues , including oocyte ateophy , degradation granulose cells , and karyopyknotic granulose lutein cells in the experimental groups .Conclusion Ephedrine has a great effect on the ovarian histological structure and ovarian function of female mice , which may be correlated with the low activities of SOD .