CAJ | 학술논문

目的：用瘤细胞悬液和瘤组织块原位种植，建立SD大鼠原位移植胃癌模型。方法直接注射法组将walker-256细胞悬液直接注射到SD大鼠胃壁的浆膜内，观察移植肿瘤的生长状况、移植成功率；组织插块法组以walker-256细胞接种于SD幼鼠皮下，形成稳定传代的皮下移植瘤，再取该肿瘤组织块原位移植于SD大鼠胃壁，观察比较移植肿瘤的生长状况、移植成功率。结果直接注射组原位移植成功率为71.42％，造模九天后平均瘤重为0.322 g；组织插块组原位移植胃癌成功率为42.85％，造模九天后平均瘤重为0.206 g。两组间比较有统计学意义（ P＜0.01）。结论该SD大鼠直接注射法原位移植模型的生物学行为与人胃癌自然生长程相似，可作为一种有价值的工具用于胃癌转移机理和抗转移实验。
목적：용류세포현액화류조직괴원위충식，건립SD대서원위이식위암모형。방법직접주사법조장walker-256세포현액직접주사도SD대서위벽적장막내，관찰이식종류적생장상황、이식성공솔；조직삽괴법조이walker-256세포접충우SD유서피하，형성은정전대적피하이식류，재취해종류조직괴원위이식우SD대서위벽，관찰비교이식종류적생장상황、이식성공솔。결과직접주사조원위이식성공솔위71.42％，조모구천후평균류중위0.322 g；조직삽괴조원위이식위암성공솔위42.85％，조모구천후평균류중위0.206 g。량조간비교유통계학의의（ P＜0.01）。결론해SD대서직접주사법원위이식모형적생물학행위여인위암자연생장정상사，가작위일충유개치적공구용우위암전이궤리화항전이실험。
Objective Tumor tissue and tumor cell suspension was inoculated into SD rats to establish orthotopic gastric cancer model.Methods Walker-256 cells were inoculated subcutaneously into SD rats ,then intact tumor tissue was collected and implanted orthotopically into SD rat stomach , the model was named indrect gastric orthotopic transplantation .To observe the tumor growth condi-tions and the success rate of transplantation .Walker-256 cell suspension will be directly injected into the stomach wall serous membrane of SD rats in direct injecrion group ,tumor growth conditions and the success rate of transplantation were observed .Results Situ trans-plant success rate in direct group was 71.42%, nine days after modeling , the average tumor weight was 0.322 g;situ tissue transplant success rate of indirect gastric orthotopic transplantation group was 42.85%,nine days after modeling , the average tumor weight was 0. 206 g.Conclusion Direct injection of the SD rat model of orthotopic transplantation is similar to human gastric cancer biological be -havior and natural growth process ,it can be used as a valuable tool for the transfer mechanism of gastric cancer and anti -transfer ex-periments .