CAJ | 학술논문

目的构建喉癌（Hep-2）细胞的酵母双杂交cDNA文库,为筛选包涵体膜蛋白分子的作用配体提供前期工作基础。方法从Hep-2细胞中提取总RNA,应用SMARTTM技术,构建以pGADT7-Rec为载体的Hep-2细胞酵母GAL4AD融合cDNA文库。结果所构建的文库展现出良好的多态性。结论可用于酵母双杂交系统的筛选。
목적구건후암（Hep-2）세포적효모쌍잡교cDNA문고,위사선포함체막단백분자적작용배체제공전기공작기출。방법종Hep-2세포중제취총RNA,응용SMARTTM기술,구건이pGADT7-Rec위재체적Hep-2세포효모GAL4AD융합cDNA문고。결과소구건적문고전현출량호적다태성。결론가용우효모쌍잡교계통적사선。
Objective To construct yeast two-hybrid cDNA library for Hep-2 cells to screen GAL4 fusion,and provide foundation for screening ligands of Chlamydia Inclusion membrane proteins.Methods The yeast GAL4 fusion cDNA library from Hep-2 cells was constructed.Total RNA of Hep-2 cells was purified by Trizol reagents,and cDNAs of the mRNAs contained in purified total RNA sample were synthesized by SMARTTM technology,which were subsequently cloned into plasmid pGADT7-Rec.Results The library showed excellent polymorphism.Conclusion The constructed librar y could be used for yeast two-hybrid system.